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[The detection of rotavirus RNA in nasopharyngeal smears by molecular hybridization].

作者信息

Novikova N A, Al'tova E E, Noskova N V, Epifanova N V, Tamoĭkina N A

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1991 Apr(4):23-5.

PMID:1652872
Abstract

Rotavirus RNA was detected in nasopharyngeal washings, obtained from patients with rotavirus gastroenteritis (76.3% of cases) and gastroenteritis of unknown etiology (34.7% of cases), by the method of molecular hybridization with the use of RNA of monkey virus SA11, labeled with 32P in the process of T4-ligase reaction. In 100% of cases this detection correlated with the presence of lesions of the upper respiratory ways and was probably indicative of the rotavirus nature of the catarrhal syndrome in rotavirus gastroenteritis.

摘要

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