Zangaladze E D, Eremeev T P, Ginevskaia V A
Vopr Virusol. 1990 Nov-Dec;35(6):500-2.
The etiology of enteric illnesses occurring during hospitalization in children admitted in January-May, 1985, for respiratory tract illnesses was studied by several methods including electron microscopy (EM), enzyme immunoassay (EIA), and PAG electrophoresis. Rotaviruses were detected in 22 (44.9%) t of 49 children with symptoms of intestinal infection, in February in 11 (84.6%) out of 13 patients. The analysis of virus genome RNA of 7 isolates positive in EM and EIA revealed in all the isolates an identical pattern of RNA segment distribution typical of "long" variants of group A rotaviruses. The appurtenance of the isolates to the same electrophoretic type together with epidemiological data allows the examined cases of rotavirus gastroenteritis to be considered as nosocomial ones. A sporadic case of gastroenteritis is described induced by a rotavirus antigenically different from Group A rotaviruses and having the electrophoretic type similar to that described for group C rotaviruses.
采用包括电子显微镜（EM）、酶免疫测定（EIA）和聚丙烯酰胺凝胶电泳（PAG电泳）在内的多种方法，对1985年1月至5月因呼吸道疾病入院的儿童住院期间发生的肠道疾病病因进行了研究。在49例有肠道感染症状的儿童中，有22例（44.9%）检测到轮状病毒，2月份13例患者中有11例（84.6%）检测到。对7株在EM和EIA中呈阳性的分离株的病毒基因组RNA分析显示，所有分离株中RNA片段分布模式相同，这是A组轮状病毒“长”变体的典型特征。分离株属于同一电泳类型，再结合流行病学数据，可将所检查的轮状病毒胃肠炎病例视为医院感染病例。本文描述了1例散发性胃肠炎病例，由一种抗原性不同于A组轮状病毒且电泳类型与C组轮状病毒相似的轮状病毒引起。
