一种用于检测轮状病毒的斑点杂交试验。

A dot hybridisation assay for detection of rotavirus.

作者信息

Flores J, Boeggeman E, Purcell R H, Sereno M, Perez I, White L, Wyatt R G, Chanock R M, Kapikian A Z

出版信息

Lancet. 1983 Mar 12;1(8324):555-8. doi: 10.1016/s0140-6736(83)92811-8.

Abstract

A "dot" hybridisation technique for the detection of rotavirus in stools and other biological materials is described. The assay is based on the in-situ hybridisation of labelled single-stranded RNA probes, obtained by in-vitro transcription of rotavirus particles, to heat-denatured rotavirus RNA immobilised on nitrocellulose membranes. The method is highly specific and allows for the detection of as little as 8 pg of viral RNA. Its use for the detection of rotavirus in stool suspensions and rectal swabs obtained from children with diarrhoea may facilitate epidemiological studies of rotavirus gastroenteritis.

摘要

本文描述了一种用于检测粪便及其他生物材料中轮状病毒的“斑点”杂交技术。该检测方法基于标记的单链RNA探针的原位杂交，这些探针通过轮状病毒颗粒的体外转录获得，与固定在硝酸纤维素膜上经热变性的轮状病毒RNA杂交。该方法具有高度特异性，能够检测低至8 pg的病毒RNA。将其用于检测腹泻儿童粪便悬液和直肠拭子中的轮状病毒，可能有助于轮状病毒性肠胃炎的流行病学研究。