过氧化物酶催化的脑啡肽氧化反应。
The peroxidase-catalyzed oxidation of enkephalins.
作者信息
Rosei M A, Blarzino C, Foppoli C, Coccia R, De Marco C
机构信息
Dipartimento di Scienze Biochimiche, Università La Sapienza Rome, Italy.
出版信息
Biochem Biophys Res Commun. 1991 Aug 30;179(1):147-52. doi: 10.1016/0006-291x(91)91347-f.
In vitro experiments are reported showing that Leu-enkephalin and Metenkephalin, in the presence of hydrogen peroxide, can be oxidized by horseradish peroxidase. The products formed are strongly fluorescent and characterized by absorption peaks with maxima at 290 nm and 315 nm. The effects of substrate and enzyme concentrations on the oxidation rate of enkephalins are described. Amino acid analysis of the hydrolysates from peroxidase-treated enkephalins provides evidence for the presence of dityrosine. The data suggest that the oxidation leads to the production of enkephalin dimers with a linkage between the N-terminal tyrosine residues. Data are also obtained indicating that enkephalins function as hydrogen donors for mammalian peroxidases.
据报道，体外实验表明，亮氨酸脑啡肽和甲硫氨酸脑啡肽在过氧化氢存在的情况下，可被辣根过氧化物酶氧化。形成的产物具有强烈的荧光，其特征在于吸收峰的最大值在290nm和315nm处。描述了底物和酶浓度对脑啡肽氧化速率的影响。对经过氧化物酶处理的脑啡肽水解产物的氨基酸分析为二酪氨酸的存在提供了证据。数据表明，氧化导致产生在N端酪氨酸残基之间有连接的脑啡肽二聚体。还获得的数据表明，脑啡肽可作为哺乳动物过氧化物酶的氢供体。
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