Han Ki-Ho, Han Arum, Frazier A Bruno
School of Electrical and Computer Engineering, Georgia Institute of Technology, 777 Atlantic Drive, Atlanta, GA 30332, USA.
Biosens Bioelectron. 2006 Apr 15;21(10):1907-14. doi: 10.1016/j.bios.2006.01.024. Epub 2006 Mar 10.
This paper presents the development of a microsystem for separating suspended breast cancer cells in peripheral blood and for sorting them based on their electrophysiological characteristics. A continuous paramagnetic capture mode (PMC) magnetophoretic microseparator was utilized for the isolation of suspended breast cancer cells in peripheral blood based on the native magnetic properties of blood cells without any tagging such as with magnetic probes. A micro-electrical impedance spectroscopy (mu-EIS) system was used as a downstream cell analysis tool to extract the pathological characteristics from the breast cancer cells. The system was fabricated on silicon and glass substrates utilizing microfabrication and stereolithography technologies. The experimental results of the PMC microseparator show that 94.8% of the breast cancer cells could be continuously separated out from a spiked blood sample with a 0.2 T external magnetic flux. The electrical impedances of human breast cancer cell lines of different pathological stages (MCF-7, MDA-MB-231, and MDA-MB-435) were measured using mu-EIS and compared to those of normal human breast tissue cell line MCF-10A.
本文介绍了一种用于分离外周血中悬浮乳腺癌细胞并根据其电生理特征进行分选的微系统的开发。一种连续顺磁捕获模式（PMC）磁泳微分离器被用于基于血细胞的天然磁性特性来分离外周血中的悬浮乳腺癌细胞，无需任何诸如磁性探针之类的标记。微电阻抗光谱（μ-EIS）系统用作下游细胞分析工具，以从乳腺癌细胞中提取病理特征。该系统利用微加工和立体光刻技术在硅和玻璃基板上制造。PMC微分离器的实验结果表明，在0.2 T外部磁通量下，94.8%的乳腺癌细胞能够从加标的血液样本中被连续分离出来。使用μ-EIS测量了不同病理阶段的人乳腺癌细胞系（MCF-7、MDA-MB-231和MDA-MB-435）的电阻抗，并与正常人乳腺组织细胞系MCF-10A的电阻抗进行了比较。
