基于电特性的HT-29结肠癌细胞电操纵与评估

HT-29 Colon Cancer Cell Electromanipulation and Assessment Based on Their Electrical Properties.

作者信息

Olariu Marius Andrei, Tucureanu Catalin, Filip Tudor Alexandru, Caras Iuliana, Salageanu Aurora, Vasile Valentin, Avram Marioara, Tincu Bianca, Turcan Ina

机构信息

Department of Electrical Measurements and Materials, Faculty of Electrical Engineering and Information Technology, Gheorghe Asachi Technical University of Iasi, 700050 Iasi, Romania.

Cantacuzino, National Medical-Military Institute for Research and Development, 050096 Bucharest, Romania.

出版信息

Micromachines (Basel). 2022 Oct 27;13(11):1833. doi: 10.3390/mi13111833.

DOI:10.3390/mi13111833

PMID:36363854

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9698395/

Abstract

This study proposes a feasible approach for the rapid, sensitive, and label-free identification of cancerous cells based on dielectrophoretic (DEP) manipulation and electrical characterization. In this method, the concentration of target cells at the level of customized microelectrodes via DEP is first determined, followed by an electrical impedance evaluation. The study demonstrates the capacity of the methodology to electrically differentiate HT-29 cancer cells from healthy blood cells based on their impedance spectra. Within a higher frequency domain, the electrical impedance of trapped cancer cells was significantly lower compared with the normal ones. In order to evaluate the functionality and reproducibility of the proposed method, the influence of the DEP and EIS (electrical impedance spectroscopy) operating voltages on the electrical characterization of trapped HT-29 cells was analyzed.

摘要

本研究提出了一种基于介电泳（DEP）操控和电学表征的快速、灵敏且无标记识别癌细胞的可行方法。在该方法中，首先通过DEP确定定制微电极水平上靶细胞的浓度，然后进行电阻抗评估。该研究证明了该方法基于阻抗谱从健康血细胞中电区分HT-29癌细胞的能力。在较高频率域内，捕获的癌细胞的电阻抗明显低于正常细胞。为了评估所提方法的功能和可重复性，分析了DEP和电阻抗谱（EIS）工作电压对捕获的HT-29细胞电学表征的影响。