硫酸阿糖聚糖衍生的大寡糖的制备与结构测定
Preparation and structural determination of large oligosaccharides derived from acharan sulfate.
作者信息
Chi Lianli, Munoz Eva M, Choi Hyung Seok, Ha Young Wan, Kim Yeong Shik, Toida Toshihiko, Linhardt Robert J
机构信息
Department of Chemistry and Chemical Biology, Chemical and Biological Engineering, and Biology, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
出版信息
Carbohydr Res. 2006 May 22;341(7):864-9. doi: 10.1016/j.carres.2006.02.030. Epub 2006 Mar 10.
Abstract
The structures of a series of large oligosaccharides derived from acharan sulfate were characterized. Acharan sulfate is an unusual glycosaminoglycan isolated from the giant African snail, Achatina fulica. Oligosaccharides from decasaccharide to hexadecasaccharide were enzymatically prepared using heparin lyase II and purified. Capillary electrophoresis and gel electrophoresis confirmed the purity of these oligosaccharides. Their structures, determined by ESI-MS and NMR, were consistent with the major repeating sequence in acharan sulfate, -->4)-alpha-d-GlcN(p)Ac-(1-->4)-alpha-l-IdoA(p)2S-(1-->, terminated by 4-linked alpha-d-GlcN(p)Ac residue at the reducing end and by 4,5-unsaturated pyranosyluronic acid 2-sulfate at the non-reducing end.
摘要
对一系列源自硫酸乙酰肝素的大寡糖的结构进行了表征。硫酸乙酰肝素是一种从巨型非洲蜗牛（褐云玛瑙螺）中分离出的特殊糖胺聚糖。使用肝素裂解酶II酶法制备了从十糖到十六糖的寡糖并进行了纯化。毛细管电泳和凝胶电泳证实了这些寡糖的纯度。通过电喷雾电离质谱（ESI-MS）和核磁共振（NMR）确定的它们的结构与硫酸乙酰肝素中的主要重复序列一致，即→4)-α-d-葡萄糖胺（N-乙酰基）-(1→4)-α-l-艾杜糖醛酸2-硫酸酯-(1→，在还原端由4-连接的α-d-葡萄糖胺（N-乙酰基）残基终止，在非还原端由4,5-不饱和吡喃糖醛酸2-硫酸酯终止。
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