Boisson Bertrand, Jacques Jean Claude, Choumet Valérie, Martin Estelle, Xu Jiannong, Vernick Ken, Bourgouin Catherine
Unité de Biologie et Génétique du Paludisme, Institut Pasteur, 28 Rue du Dr Roux 75724 Paris Cedex, France.
FEBS Lett. 2006 Apr 3;580(8):1988-92. doi: 10.1016/j.febslet.2006.02.069. Epub 2006 Mar 6.
Salivary glands are the ultimate site of development in the insect of mosquito born pathogens such as Plasmodium. Mosquito salivary glands also secrete components involved in anti-haemostatic activities and allergic reactions. We investigated the feasibility of RNAi as a tool for functional analysis of genes expressed in Anopheles gambiae salivary glands. We show that specific gene silencing in salivary glands requires the use of large amounts of dsRNA, condition that differs from those for efficient RNAi in other mosquito tissues. Using this protocol, we demonstrated the role of AgApy, which encodes an apyrase, in the probing behaviour of An. gambiae.
唾液腺是疟原虫等蚊媒病原体在昆虫体内发育的最终部位。蚊唾液腺还分泌参与抗止血活动和过敏反应的成分。我们研究了RNA干扰作为冈比亚按蚊唾液腺中表达基因功能分析工具的可行性。我们发现，唾液腺中的特定基因沉默需要使用大量双链RNA，这一条件与其他蚊组织中有效RNA干扰的条件不同。利用该方案，我们证明了编码腺苷三磷酸双磷酸酶的AgApy在冈比亚按蚊刺探行为中的作用。
