Arcà B, Lombardo F, Capurro M, della Torre A, Spanos L, Dimopoulos G, Louis C, James A A, Coluzzi M
Istituto di Parassitologia, Fondazione Istituto Pasteur-Cenci Bolognetti, Università La Sapienza, Roma, Italy.
Parassitologia. 1999 Sep;41(1-3):483-7.
Molecular studies on the tissue-specific gene expression in the salivary glands of Anopheles gambiae may provide useful tools for the development of new strategies for the control of the most efficient malaria vector in the sub-Saharan Africa. We summarize here the results of a recent investigation focused on the isolation of secreted factors and putative receptors from the salivary glands of An. gambiae. Using the Signal Sequence Trap technique we have identified the first cDNAs specifically expressed in the An. gambiae salivary glands. Among these, four are exclusively expressed in female glands and encode factors presumably involved in blood-feeding, whereas two other cDNAs seem to be expressed both in male and in female glands and are likely implicated in sugar-feeding. Homologues of genes previously identified in the yellow fever mosquito Aedes aegypti, like the apyrase and D7, as well as novel salivary gland-specific cDNAs, were identified. The isolation and characterization of promoter sequences from the corresponding genes may prove useful for the expression of anti parasitic agents in the salivary glands of transgenic mosquitoes.
对冈比亚按蚊唾液腺组织特异性基因表达的分子研究，可能为制定新策略来控制撒哈拉以南非洲地区最有效的疟疾传播媒介提供有用工具。我们在此总结了最近一项调查的结果，该调查聚焦于从冈比亚按蚊唾液腺中分离分泌因子和假定受体。利用信号序列捕获技术，我们鉴定出了首批在冈比亚按蚊唾液腺中特异性表达的cDNA。其中，有四个仅在雌蚊腺体中表达，编码的因子可能参与吸血过程；而另外两个cDNA似乎在雄蚊和雌蚊腺体中均有表达，可能与取食糖类有关。我们还鉴定出了先前在黄热病蚊子埃及伊蚊中发现的基因同源物，如腺苷三磷酸双磷酸酶和D7，以及新的唾液腺特异性cDNA。从相应基因中分离和表征启动子序列，可能对在转基因蚊子的唾液腺中表达抗寄生虫剂有用。
