Radács M, Gálfi M, Juhász A, Varga Cs, Molnár A, László F, László F A
Department of Biology, Faculty of Juhász Gyula Teachers Training College, University of Sciences, Szeged, Hungary.
Regul Pept. 2006 May 15;134(2-3):82-8. doi: 10.1016/j.regpep.2006.01.006. Epub 2006 Mar 10.
The effects of histamine (HA) on vasopressin (VP) and oxytocin (OT) secretion were studied in 13-14-day cultures of isolated rat neurohypophyseal (NH) tissue. The VP and OT contents of the supernatant were determined by radioimmunoassay (RIA) after a 1 or 2-h incubation. Significantly increased levels of VP and OT production were detected in the tissue culture media following HA administration, depending on the HA dose. The elevation of NH hormone secretion could be partially blocked by previous administration of the HA antagonist mepyramine (MEP, an H1 receptor antagonist) or cimetidine (CIM, an H2 receptor antagonist). Thioperamide (TPE, an H3-H4 receptor antagonist) did not influence the VP or OT secretion increase induced by HA. The application of MEP, CIM or TPE after HA administration proved ineffective. The H1 and H2 receptors are mainly involved in the HA-induced increase of both VP and OT secretion in isolated NH tissue cultures. The results indicate that NH hormone release is influenced directly by the histaminergic system, and the histaminergic control of VP and OT secretion from the NH tissue in rats can occur at the level of the posterior pituitary.
在分离的大鼠神经垂体（NH）组织的13 - 14天培养物中研究了组胺（HA）对血管加压素（VP）和催产素（OT）分泌的影响。孵育1或2小时后，通过放射免疫测定法（RIA）测定上清液中的VP和OT含量。给予HA后，根据HA剂量的不同，在组织培养基中检测到VP和OT产生水平显著升高。预先给予HA拮抗剂美吡拉敏（MEP，一种H1受体拮抗剂）或西咪替丁（CIM，一种H2受体拮抗剂）可部分阻断NH激素分泌的升高。硫代酰胺（TPE，一种H3 - H4受体拮抗剂）不影响HA诱导的VP或OT分泌增加。HA给药后应用MEP、CIM或TPE证明无效。H1和H2受体主要参与HA诱导的分离的NH组织培养物中VP和OT分泌的增加。结果表明，NH激素释放直接受组胺能系统影响，并且大鼠NH组织中VP和OT分泌的组胺能控制可发生在垂体后叶水平。
