Department of Biology, Gyula Juhász Faculty of Education, University of Szeged, Boldogasszony sgt. 6., Szeged 6725, Hungary.
J Mol Neurosci. 2010 Sep;42(1):59-66. doi: 10.1007/s12031-010-9331-3. Epub 2010 Feb 10.
The effects of the interactions between the 29 amino acid-containing peptide galanin and adrenaline or noradrenaline on the secretion of oxytocin were studied in 13- to 14-day cultures of isolated rat neurohypophyseal tissue. The alpha-receptor antagonist corynanthine blocked the adrenaline-induced increase of oxytocin secretion. When the beta-receptor antagonist propranolol was added before the noradrenaline treatment, the antagonist prevented the noradrenaline-induced enhancement of oxytocin release. Following the addition of galanin, the extent of oxytocin secretion into the supernatant medium decreased. Adrenaline and noradrenaline treatments increased the oxytocin level. Preincubation with galanin reduced the adrenaline- and noradrenaline-induced oxytocin level elevations. The blocking effect of galanin was prevented by previous treatment with the galanin receptor antagonist galantid (M15). When adrenaline or noradrenaline treatment was applied before galanin addition, the oxytocin secretion remained enhanced. The present results indicate that the changes in oxytocin secretion induced by the adrenergic system can be directly influenced by the galaninergic system. The interactions between the adrenergic and galaninergic systems from the aspect of oxytocin secretion can occur at the level of the posterior pituitary, independently of the hypothalamus.
在 13-14 天龄的离体大鼠神经垂体组织培养物中，研究了含有 29 个氨基酸的肽甘丙肽与肾上腺素或去甲肾上腺素相互作用对催产素分泌的影响。α-受体拮抗剂可乐宁阻断了肾上腺素引起的催产素分泌增加。当β-受体拮抗剂普萘洛尔在去甲肾上腺素处理前加入时，该拮抗剂阻止了去甲肾上腺素引起的催产素释放增强。甘丙肽添加后，催产素分泌到上清液中的程度下降。肾上腺素和去甲肾上腺素处理增加了催产素水平。甘丙肽的预孵育降低了肾上腺素和去甲肾上腺素引起的催产素水平升高。甘丙肽受体拮抗剂甘丙肽 (M15) 的预处理可防止甘丙肽的阻断作用。当在添加甘丙肽之前应用肾上腺素或去甲肾上腺素处理时，催产素分泌仍然增强。这些结果表明，儿茶酚胺系统引起的催产素分泌变化可以直接受到甘丙肽能系统的影响。从催产素分泌的角度来看，儿茶酚胺系统和甘丙肽系统之间的相互作用可以在后垂体水平发生，而与下丘脑无关。
