Maslow D E, Mayhew E
J Natl Cancer Inst. 1975 May;54(5):1097-102. doi: 10.1093/jnci/54.5.1097.
The effects of normal and malignant cells on the aggregation of embryonic cells in gyratory shaker cultures were compared. The addition of 1 times 10-5 simian virus 40 (SV40)-transformed BALB/3T3 (SV40-3T3) cells to 6 times 10-6 embryonic neural retina cells caused a highly significant greater reduct on (22.7 percent) in aggregate diameter than the addition of untransformed BALB/3T3 (3T3) cells. The ratio of the number of single cells to the number of aggregates was significantly higher for cultures containing SV40-3T3 cells than for the cultures containing 3T3 cells. This effect was concentration dependent in the presence of cultured Ehrlich-Lettre hyperdiploid (ELD) ascites cells; however, media from ELD cell cultures or ELD cell sonicates resulted in aggregates of greater diameter and lower ratios of single cells to aggregates. This approach may provide a sensitive assay system for the interactions of tumor and other cells in vitro.
比较了正常细胞和恶性细胞对旋转振荡器培养中胚胎细胞聚集的影响。向6×10⁻⁶个胚胎神经视网膜细胞中添加1×10⁻⁵个猴病毒40（SV40）转化的BALB/3T3（SV40 - 3T3）细胞，与添加未转化的BALB/3T3（3T3）细胞相比，聚集体直径的减小幅度显著更大（22.7%）。含有SV40 - 3T3细胞的培养物中单个细胞数量与聚集体数量的比率显著高于含有3T3细胞的培养物。在培养的艾氏腹水瘤超二倍体（ELD）细胞存在的情况下，这种效应具有浓度依赖性；然而，ELD细胞培养物的培养基或ELD细胞超声裂解物导致形成直径更大且单个细胞与聚集体比率更低的聚集体。这种方法可能为体外肿瘤细胞与其他细胞的相互作用提供一种灵敏的检测系统。
