Smets L A, Homburg C, Van Rooy H
Cell Biol Int Rep. 1979 Mar;3(2):107-111. doi: 10.1016/0309-1651(79)90114-0.
Explanted tumour cells are much more sensitive to the deleterious effects of routine trypsinization than are the parent 3T3 or SV40 transformed established cell lines. This differential sensitivity causes the disappearance of tumour-derived cells when grown in co-culture with untransformed 3T3 cells and accounts in some tumor explants for the emergence of trypsin-resistant varient cells which have lost tumour-specific properties.
与亲代3T3细胞或SV40转化的已建立细胞系相比，移植的肿瘤细胞对常规胰蛋白酶消化的有害作用更为敏感。这种差异敏感性导致肿瘤衍生细胞在与未转化的3T3细胞共培养时消失，并在一些肿瘤外植体中导致失去肿瘤特异性特性的胰蛋白酶抗性变异细胞的出现。
