Daniel L, Fakhouri F, Joly D, Mouthon L, Nusbaum P, Grunfeld J-P, Schifferli J, Guillevin L, Lesavre P, Halbwachs-Mecarelli L
Department of Pathology, CHU Timone and EA 3281, IFR 125, Faculty of Medicine, Marseille, France.
Kidney Int. 2006 Apr;69(8):1416-23. doi: 10.1038/sj.ki.5000306.
Release of microparticles (MPs) from blood cells may occur upon various activation signals. MPs from neutrophil and platelet have been studied in systemic infectious diseases and cardiovascular diseases, respectively. They are here investigated in common nephropathies including vasculitis and dialysis, two conditions characterized by neutrophil activation. Flow cytometry analysis of neutrophil-derived (CD66b-positive) and platelet-derived (CD41a-positive) MPs was performed on 213 plasma samples from patients with various nephropathies, including 46 patients with vasculitis and 40 hemodialysis patients. MPs released ex vivo, during neutrophil activation in whole blood, were also measured in these patients. Correlations with clinical parameters and creatinine clearance were evaluated. The results show that MPs present in plasma from patients or healthy controls are from various origins: platelet-derived (38+/-22%), neutrophil-derived (2.8+/-3.8%) MPs, mixed aggregates of neutrophil/platelet MPs (28+/-15%) or neither from neutrophil or platelet (null) 31+/-20%. Acute vasculitis showed the highest level of all types of MPs, while other nephropathies did not result in significant changes of MP levels. A significant increase was observed during hemodialysis sessions. In patients with renal failure, no correlation was seen between MP levels and creatinine clearance. In conclusion, neutrophil and platelet MP levels are non-specific markers of neutrophil activation during vasculitis acute phase and dialysis-induced inflammation. Circulating aggregates of neutrophil/platelet MPs co-express adhesion molecules of both cell types and may be thus endowed with inflammation and coagulation- thus modulating properties.
血细胞释放微粒（MPs）可能发生于各种激活信号作用下。来自中性粒细胞和血小板的MPs已分别在系统性感染性疾病和心血管疾病中得到研究。本文对包括血管炎和透析在内的常见肾病中的MPs进行了研究，这两种病症的特征均为中性粒细胞激活。对来自各种肾病患者的213份血浆样本进行了流式细胞术分析，以检测源自中性粒细胞（CD66b阳性）和血小板（CD41a阳性）的MPs，其中包括46例血管炎患者和40例血液透析患者。还检测了这些患者全血中中性粒细胞激活过程中离体释放的MPs。评估了其与临床参数及肌酐清除率的相关性。结果显示，患者或健康对照血浆中的MPs来源各异：源自血小板的（38±22%）、源自中性粒细胞的（2.8±3.8%）MPs、中性粒细胞/血小板MPs的混合聚集体（28±15%）或既非源自中性粒细胞也非源自血小板的（无）31±20%。急性血管炎患者所有类型的MPs水平最高，而其他肾病并未导致MPs水平发生显著变化。血液透析过程中MPs水平显著升高。在肾衰竭患者中，MPs水平与肌酐清除率之间未见相关性。总之，中性粒细胞和血小板MPs水平是血管炎急性期和透析诱导炎症期间中性粒细胞激活的非特异性标志物。中性粒细胞/血小板MPs的循环聚集体共表达两种细胞类型的黏附分子，因此可能具有炎症和凝血调节特性。
