Kumar G Suresh, Vijayalakshmi B, Salimath P V
Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore 570 020, India.
Mol Cell Biochem. 2006 Jun;286(1-2):53-8. doi: 10.1007/s11010-005-9086-2. Epub 2006 Mar 11.
Diet is now one of the well established means in the management of diabetes. Bitter gourd and spent turmeric at 10% level were tested for their efficacy on glycosaminoglycan metabolism in various tissues viz., liver, spleen, lungs, heart and testis in control, diabetic and treated rats. The glycosaminoglycans (GAGs) were isolated from defatted and dried tissues. The contents of sulfated GAGs decreased in all the tissues and the decrease was more prominent in heart and testis. In the isolated GAGs, contents of total sugar, amino sugar, uronic acid and sulfate were studied. Decrease in total sugar content was maximum in testis. Amino sugar content decreased considerably in testis (38%) and lungs (15%). The content of uronic acid also decreased in testis (33%) besides heart (29%) and liver (25%). Sulfate groups in GAGs perform pivotal functions in many biological events and decrease in sulfate content was significant in heart (40%), testis (37%) and liver (37%). GAGs profile on the cellulose acetate electrophoresis revealed that heparan sulfate (HS), hyaluronic acid (HA) and chondroitin sulfate/dermatan sulfate (CS/DS) were present in liver, spleen and lungs. HS, CS were present in heart, DS/CS was observed in testis. The observed beneficial effects in GAGs metabolism during diabetes may be due to the presence of high amounts of dietary fibres present in bitter gourd and spent turmeric, besides, possible presence of bioactive compounds in one or both of them.
饮食现在是糖尿病管理中已确立的方法之一。对苦瓜和用过的姜黄以10%的水平进行了测试，观察它们对正常、糖尿病和经治疗大鼠的肝脏、脾脏、肺、心脏和睾丸等各种组织中糖胺聚糖代谢的功效。从脱脂干燥的组织中分离出糖胺聚糖（GAGs）。所有组织中硫酸化GAGs的含量均下降，且在心脏和睾丸中下降更为显著。对分离出的GAGs，研究了总糖、氨基糖、糖醛酸和硫酸盐的含量。睾丸中总糖含量下降最多。睾丸（38%）和肺（15%）中的氨基糖含量大幅下降。除心脏（29%）和肝脏（25%）外，睾丸中糖醛酸含量也下降（33%）。GAGs中的硫酸基团在许多生物事件中发挥关键作用，心脏（40%）、睾丸（37%）和肝脏（37%）中硫酸盐含量的下降显著。醋酸纤维素电泳的GAGs图谱显示，肝脏、脾脏和肺中存在硫酸乙酰肝素（HS）、透明质酸（HA）和硫酸软骨素/硫酸皮肤素（CS/DS）。心脏中存在HS、CS，睾丸中观察到DS/CS。糖尿病期间在GAGs代谢中观察到的有益作用可能是由于苦瓜和用过的姜黄中存在大量膳食纤维，此外，它们中的一种或两种可能存在生物活性化合物。
