[Studies on the preparation and characterization of immobilized neutral protease by carboxymethyl chitosan microsphere].
作者信息
Jin Zhitao, Chen Guohua, Liu Xiaoyun, Zhao Yu, Chen Yao, Gao Congjie
机构信息
Department of Chemistry and Chemical Engineering of Ocean University of China, Qingdao 266003, China.
出版信息
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2006 Feb;23(1):97-101.
PMID:16532820
Abstract
AS1. 398 neutral protease was immobilized onto carboxymethyl chitosan with glutaraldehyde as cross-linking agent. The effects of pH, time of cross-linking, amount of cross-linking agent and the ratio of enzyme to carrier on the activity of the immobilized enzyme were demonstrated, and the optimum immobilization condition of AS1. 398 neutral protease was established. Also studied was the characterization of immobilized enzyme,including pH, temperature, Km and the stability of storage.
摘要
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