Larghero Jerome, Rea Delphine, Brossard Yves, Van Nifterik Jacqueline, Delasse Veronique, Robert Isabelle, Biscay Nicole, Chantre Elisabeth, Raffoux Emmanuel, Socié Gerard, Gluckman Eliane, Benbunan Marc, Marolleau Jean-Pierre
Cell Therapy Unit and Bone Marrow Transplant Department, Saint-Louis Hospital, Paris, France.
Transfusion. 2006 Mar;46(3):403-6. doi: 10.1111/j.1537-2995.2006.00736.x.
Cord blood (CB) represents an alternate source of stem cells in transplantation. Nucleated red blood cells (NRBCs) are a physiological subset of CB population. Although it is important to have an accurate estimate of CD34(+) cell number, NRBCs could compromise white blood cell count and interfere with CD34(+) cell quantification.
A total of 826 CB units were analyzed for total nucleated cells (TNCs), NRBCs, and CD34(+) cells by flow cytometry. NRBCs were also counted conventionally by manual microscopy. Percentages of CD34(+) cells corrected by NRBC count (CD34+c) were determined as follows: %CD34+c = CD34(+)/CD45(+) (x10(6))/(TNCs (x10(8)) - NRBCs (x10(8))).
The mean percentages of CD34+ cells and NRBCs were 0.27 percent (range, 0.01%-1.25%) and 7.64 percent (range, 0.13%-84%), respectively. Comparison between flow cytometric and microscopic NRBC count showed a regression of y = 0.685 + 0.719x and a coefficient of determination of r(2) = 0.721. When corrected with NRBC count, the mean percentage of CD34(+) c cells was 0.295 percent (p = 0.0008 compared with CD34(+)%) and mean TNCc count was 14.8 x 10(8) (p < 10(-4) compared to TNC count).
The determination of NRBCs with a flow cytometric method might represent a new strategy for providing satisfactory quality assurance controls of CB products.
脐血（CB）是移植中干细胞的另一种来源。有核红细胞（NRBCs）是CB群体的一个生理亚群。虽然准确估计CD34(+)细胞数量很重要，但NRBCs可能会影响白细胞计数并干扰CD34(+)细胞定量。
通过流式细胞术对总共826个CB样本进行了总核细胞（TNCs）、NRBCs和CD34(+)细胞分析。NRBCs也通过手动显微镜进行常规计数。经NRBC计数校正的CD34(+)细胞百分比（CD34+c）的计算方法如下：%CD34+c = CD34(+)/CD45(+)（×10(6)）/（TNCs（×10(8)） - NRBCs（×10(8)））。
CD34+细胞和NRBCs的平均百分比分别为0.27%（范围为0.01%-1.25%）和7.64%（范围为0.13%-84%）。流式细胞术和显微镜下NRBC计数之间的比较显示回归方程为y = 0.685 + 0.719x，决定系数r(2) = 0.721。经NRBC计数校正后，CD34(+) c细胞的平均百分比为0.295%（与CD34(+)%相比，p = 0.0008），平均TNCc计数为14.8×10(8)（与TNC计数相比，p < 10(-4)）。
采用流式细胞术测定NRBCs可能是为脐血产品提供令人满意的质量保证控制的一种新策略。
