通过荧光原位杂交将11个功能基因定位到鸡染色体的细胞遗传学图谱。

Cytogenetic mapping of 11 functional genes to chicken chromosomes by fluorescence in situ hybridization.

作者信息

Yuan J, Zhao Y, Zhang L, Li N

机构信息

Department of Biochemistry and Molecular Biology, State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing.

出版信息

J Anim Breed Genet. 2006 Apr;123(2):136-40. doi: 10.1111/j.1439-0388.2006.00571.x.

DOI:10.1111/j.1439-0388.2006.00571.x

PMID:16533368

Abstract

With chicken bacterial artificial chromosome (BAC) DNA as probes, 11 non-assigned functional genes were localized to chicken chromosomes 1 or 2 by fluorescence in situ hybridization (FISH). The 11 genes and their chromosomal positions are as follows: ALVEB5, 1p26-24; ACO2, 1p16-14; HSP108, 1p14-13; CD4, 1q11; HSD3B; 1q11; SOD1, 1q14-21; LAMP1, 1q24-31; P2Y5, 1q35-36; EN2, 2p31-24; NPY, 2p14-13 and CA2, 2q31-32. Metaphase chromosome spreads used for hybridization were prepared from embryonic chicken fibroblast cultures. The gene position was identified according to the international standardized G-banded karyotype of chicken by measuring the relative fractional length from the telomere of the p-arm to the hybridization signal (FLpter). The 11 genes mapped newly will enrich the cytogenetic map and serve as additional anchor markers for integrating the cytogenetic map with the genetic map of chicken.

摘要

以鸡细菌人工染色体（BAC）DNA为探针，通过荧光原位杂交（FISH）将11个未定位的功能基因定位到鸡的1号或2号染色体上。这11个基因及其染色体位置如下：ALVEB5，1p26 - 24；ACO2，1p16 - 14；HSP108，1p14 - 13；CD4，1q11；HSD3B，1q11；SOD1，1q14 - 21；LAMP1，1q24 - 31；P2Y5，1q35 - 36；EN2，2p31 - 24；NPY，2p14 - 13；CA2，2q31 - 32。用于杂交的中期染色体铺片取自鸡胚成纤维细胞培养物。通过测量从p臂端粒到杂交信号的相对分数长度（FLpter），根据鸡的国际标准化G带核型确定基因位置。新定位的这11个基因将丰富细胞遗传图谱，并作为将细胞遗传图谱与鸡的遗传图谱整合的额外锚定标记。