山羊肌肉生长抑制素基因中的单核苷酸多态性鉴定

Single-nucleotide polymorphism identification in the caprine myostatin gene.

作者信息

Li X L, Wu Zh L, Gong Y F, Liu Y Q, Liu Z Z, Wang X J, Xin T R, Ji Q

机构信息

College of Animal Science and Technology, Hebei Agricultural University, Baoding, China.

出版信息

J Anim Breed Genet. 2006 Apr;123(2):141-4. doi: 10.1111/j.1439-0388.2006.00572.x.

DOI:10.1111/j.1439-0388.2006.00572.x

PMID:16533369

Abstract

Polymerase chain reaction (PCR) products of MSTN gene amplified from 35 goats representing 17 Chinese indigenous goat breeds and five imported goat breeds were sequenced to identify the single-nucleotide polymorphisms (SNPs) of a 379-bp fragment including part of intron 2 and exon 3 of MSTN gene. A total of eight SNPs (A1980G, G1981C, A1982G, G1984T, A2121G, T2124C, G2174A and A2246G) were identified among the sequenced goats. The SNPs found are all located in intron 2 except for A2246G, which was a synonymous mutation in exon 3. Four haplotypes were sorted from these eight SNPs, of which, haplotype I (AGAGATGA) and haplotype II (GCGTGTAA) are the two main haplotypes with the frequency of 77.8% and 14.8% respectively. The SNPs found at positions 1980, 1981, 1982, 1984 and 2121 might be linked to inheritance completely.

摘要

对来自代表17个中国本土山羊品种和5个引进山羊品种的35只山羊的肌肉生长抑制素（MSTN）基因的聚合酶链反应（PCR）产物进行测序，以鉴定包含MSTN基因部分内含子2和外显子3的379bp片段的单核苷酸多态性（SNP）。在测序的山羊中总共鉴定出8个SNP（A1980G、G1981C、A1982G、G1984T、A2121G、T2124C、G2174A和A2246G）。除A2246G外，发现的SNP均位于内含子2中，A2246G是外显子3中的同义突变。从这8个SNP中分类出4种单倍型，其中单倍型I（AGAGATGA）和单倍型II（GCGTGTAA）是两种主要单倍型，频率分别为77.8%和14.8%。在位置1980、1981、1982、1984和2121处发现的SNP可能与遗传完全连锁。