Gong Y F, Li X L, Liu Z Z, Jin X M, Zhou R Y, Li L H, Zhang Q
Hebei Key Laboratory of Veterinary Preventive Medicine, Department of Animal Science, Hebei Normal University of Science & Technology, Changli, 066600, China.
Genetika. 2009 Dec;45(12):1646-9.
Polymerase chain reaction (PCR) products of the MSTN gene amplified from sixty sheep of nine Chinese indigenous sheep breeds and one imported sheep breed were sequenced to identify the single-nucleotide polymorphisms (SNPs) in a 378-bp fragment including intron 2 and exon 3 of the MSTN gene. A total of fifteen SNPs (A1937C, T1942G, C1956T, A1972C, A1990G, A2008C, A2011G, C2019T, A2025C, A2027C, T2085G, T2173C, C2198T, C2210T and C2213T) were detected among the sixty sequenced individuals and they were all located in intron 2. Twelve haplotypes were identified from these fifteen SNPs, of which haplotype I (CGTCGCGTCCGCTTT) and VIII (ATCAAAACAATTCCC) were the two major and basic ones with frequencies of 12.25% and 77.80%, respectively. Haplotype VIII was distributed in all sheep breeds and all individuals of the meat or meat-wool type sheep breeds were homozygous with respect to this haplotype. This suggests that haplotype VIII might be related to meat production traits in sheep. Haplotype I was only distributed in the fur, lambskin type and fur-meat type sheep breeds. This suggests that haplotype I may have some relationship with fur traits in sheep.
对从9个中国本土绵羊品种和1个引进绵羊品种的60只绵羊中扩增得到的MSTN基因的聚合酶链反应（PCR）产物进行测序，以鉴定MSTN基因内含子2和外显子3的378 bp片段中的单核苷酸多态性（SNP）。在60个测序个体中共检测到15个SNP（A1937C、T1942G、C1956T、A1972C、A1990G、A2008C、A2011G、C2019T、A2025C、A2027C、T2085G、T2173C、C2198T、C2210T和C2213T），它们均位于内含子2中。从这15个SNP中鉴定出12种单倍型，其中单倍型I（CGTCGCGTCCGCTTT）和VIII（ATCAAAACAATTCCC）是两个主要的基本单倍型，频率分别为12.25%和77.80%。单倍型VIII分布于所有绵羊品种，所有肉用或肉毛兼用型绵羊品种的个体对此单倍型均为纯合。这表明单倍型VIII可能与绵羊的产肉性状有关。单倍型I仅分布于裘皮、羔皮型和裘皮-肉用型绵羊品种。这表明单倍型I可能与绵羊的裘皮性状有一定关系。
