Tribollet E, Goumaz M, Raggenbass M, Dreifuss J J
Department of Physiology, University Medical Center, Geneva, Switzerland.
J Recept Res. 1991;11(1-4):333-46. doi: 10.3109/10799899109066412.
Binding sites for AVP and for OT were studied by in vitro autoradiography in sections from the brain of rat fetuses, neonates and infants; their distribution was compared to that of the brain of adults. Specific binding sites were first detected in the vagal complex for OT and in the reticular formation for AVP at E14 and E16 respectively. In the perinatal period, other areas become labeled. Approximately one week after birth, a "stable" pattern of distribution is established for AVP binding sites, and a different "stable" pattern obtained for OT binding sites. For both types of sites and in many areas, the density of labeling increases during the next two weeks to reach adult levels, whereas labeling decreases concomitantly in other areas of the brain. The distribution of AVP binding sites is of the adult pattern by the time of weaning. In contrast, the adult pattern of distribution of OT binding sites is only established after puberty, when new OT receptors appear in some regions of the hypothalamus and basal forebrain. "Transient" binding sites for AVP and OT, i.e. sites located in areas which were labeled in neonates but not in weanlings, were shown to have the same ligand affinity than the binding sites present in the adult. Electrophysiological studies suggest that at least some of these "transient" binding sites represent authentic receptors and may be involved in neuronal signaling.
通过体外放射自显影技术，研究了大鼠胎儿、新生儿和婴儿大脑切片中抗利尿激素（AVP）和催产素（OT）的结合位点；并将它们的分布与成年大鼠大脑的分布进行了比较。分别在胚胎第14天和第16天，首次在迷走神经复合体中检测到OT的特异性结合位点，在网状结构中检测到AVP的特异性结合位点。在围产期，其他区域开始出现标记。出生后约一周，AVP结合位点建立起一种“稳定”的分布模式，OT结合位点则呈现出另一种不同的“稳定”模式。对于这两种类型的位点以及许多脑区，在接下来的两周内标记密度增加至成年水平，而大脑其他区域的标记则相应减少。到断奶时，AVP结合位点的分布已呈成年模式。相比之下，OT结合位点的成年分布模式直到青春期后才建立，此时下丘脑和基底前脑的一些区域出现了新的OT受体。已证明，AVP和OT的“瞬时”结合位点，即在新生儿期有标记但在断奶期没有标记的区域中的位点，与成年大鼠中存在的结合位点具有相同的配体亲和力。电生理研究表明，这些“瞬时”结合位点中至少有一些代表真正的受体，可能参与神经元信号传导。
