Nakatsuji Y, Kiyosawa K, Tanaka E, Sodeyama T, Horigome N, Kajikawa S, Naito S, Akahane Y
Second Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto, Japan.
Liver. 1991 Jun;11(3):176-84. doi: 10.1111/j.1600-0676.1991.tb00512.x.
To clarify the discrepancy in hepatitis B surface antigen (HBsAg) subtypes present in the serum and liver, as well as among hepatocytes, liver specimens which were resected from 37 HBsAg-positive patients with hepatocellular carcinoma (HCC) were examined. We evaluated HBsAg and the subtypic determinants of HBsAg and hepatitis B core antigen (HBcAg) using the peroxidase-antiperoxidase (PAP) staining method. Hepatitis B antigens were more frequently detected in small tumors (HBsAg in 67%. HBcAg in 40%) than in large ones (HBsAg in 36%, HBcAg in 14%). The prevalence of each subtypic determinant in the HBsAg positive non-tumorous vs. tumorous areas was 100% vs. 67% in a, 100% vs. 57% in d, 100% vs. not tested in y, 100% vs. 53% in r and 25% vs. 0% in w (a, d, y, r and w represent subtypic determinants). There was virtually no difference in a set of subtypic determinants between the serum and liver. However, there were some variations in a set of subtypic determinants among the hepatocytes. On the other hand, liver tissue of compound subtype adyr in serum contained both cells with a,d,r and with a,y,r as well as a few cells with a,d,y,r. These findings suggest that HBV genomes in hepatocytes of type B chronic liver disease may differ genetically among cells even in the same liver tissue.
为了阐明血清和肝脏中以及肝细胞之间存在的乙型肝炎表面抗原（HBsAg）亚型差异，我们对37例HBsAg阳性肝细胞癌（HCC）患者切除的肝脏标本进行了检查。我们采用过氧化物酶 - 抗过氧化物酶（PAP）染色法评估HBsAg、HBsAg的亚型决定簇以及乙型肝炎核心抗原（HBcAg）。与大肿瘤（HBsAg阳性率36%，HBcAg阳性率14%）相比，小肿瘤中更频繁地检测到乙型肝炎抗原（HBsAg阳性率67%，HBcAg阳性率40%）。在HBsAg阳性的非肿瘤区域与肿瘤区域中，各亚型决定簇的流行率分别为：a型100% 对67%，d型100% 对57%，y型100% 对未检测，r型100% 对53%，w型25% 对0%（a、d、y、r和w代表亚型决定簇）。血清和肝脏中的一组亚型决定簇实际上没有差异。然而，肝细胞之间的一组亚型决定簇存在一些变化。另一方面，血清中复合亚型adyr的肝组织包含具有a、d、r以及具有a、y、r的细胞，还有少数具有a、d、y、r的细胞。这些发现表明，即使在同一肝组织中，B型慢性肝病肝细胞中的HBV基因组在细胞间可能存在基因差异。
