Oda N
Department of Surgery, Chiba University School of Medicine, Japan.
Nihon Geka Gakkai Zasshi. 1991 Jun;92(6):672-80.
The mucin alterations associated with the progress of cellular atypia of the colorectal mucosa were investigated by light and electron microscopy using lectin-histochemical technique with DBA and PNA. The samples showing positive staining in the more than one-third areas of the colonic epithelial mucosa were scored as positive reaction. The DBA positive reaction was found 100%, 95.8% and 45.7% in the normal mucosa, adenoma and carcinoma, respectively. On the other hand, the PNA positive cases increased with the grade of malignancy; 18.2%, 58.5% and 97.1% respectively. The DBA binding sites were found in the mucin of the goblet cells of the normal and adenomatous epithelia. The PNA positive sites were localized in the supranuclear portion of the normal mucosa and adenoma while in the epithelia of the carcinoma the PNA positive areas were found in the intraglandular substance and cell apex. With the electron microscopy the PNA binding sites were detected in the cis cistern of Golgi apparatus in the normal and adenomatous epithelia and in the apical membrane in the epithelia of the carcinoma. It is suggested that the PNA binding activity is a useful marker to detect the adenomas with the high risk of malignant transformation.
采用伴刀豆球蛋白A（DBA）和花生凝集素（PNA）的凝集素组织化学技术，通过光学显微镜和电子显微镜研究了与大肠黏膜细胞异型性进展相关的黏蛋白改变。在结肠上皮黏膜超过三分之一区域显示阳性染色的样本被记为阳性反应。在正常黏膜、腺瘤和癌组织中，DBA阳性反应分别为100%、95.8%和45.7%。另一方面，PNA阳性病例随恶性程度增加；分别为18.2%、58.5%和97.1%。在正常和腺瘤上皮的杯状细胞黏蛋白中发现了DBA结合位点。PNA阳性位点定位于正常黏膜和腺瘤的核上部分，而在癌上皮中，PNA阳性区域见于腺泡内物质和细胞顶端。通过电子显微镜观察，在正常和腺瘤上皮的高尔基体顺面潴泡以及癌上皮的顶端膜中检测到了PNA结合位点。提示PNA结合活性是检测具有高恶性转化风险腺瘤的有用标志物。
