Shah M, Shrikhande S S, Swaroop V S
Indian J Gastroenterol. 1989 Jan;8(1):31-3.
Lectin binding of goblet cell mucin in human colonic mucosa was studied in patients with irritable bowel syndrome, colorectal malignancy and ulcerative colitis using plant lectins, Dolichos biflorus agglutinin (DBA) and peanut agglutinin (PNA). Normal colonic mucosa demonstrated a strong binding with DBA (100%) but did not bind to PNA at all. Colonic carcinomas showed strong PNA binding (7 of 15 biopsies) while DBA binding was absent in 14 of 15 biopsies. The transitional mucosa showed reduced or absent DBA binding in 6 and positive PNA binding in 2 of 15 biopsies. During the active phase of ulcerative colitis, there was a loss of DBA binding in 10 of 15 biopsies, which was restored during remission in all. One biopsy with severe dysplasia showed PNA binding. It is concluded that normal colorectal mucosa binds DBA strongly but not PNA. Malignant tissue and transitional mucosa reveal PNA binding often, with decreased DBA binding. In ulcerative colitis DBA binding decreases during phases of activity.
利用植物凝集素双花扁豆凝集素（DBA）和花生凝集素（PNA），对肠易激综合征、结直肠癌和溃疡性结肠炎患者的人结肠黏膜杯状细胞黏蛋白的凝集素结合情况进行了研究。正常结肠黏膜与DBA呈现强烈结合（100%），但与PNA完全不结合。结肠癌显示出强烈的PNA结合（15份活检标本中有7份），而15份活检标本中有14份不存在DBA结合。移行黏膜在15份活检标本中有6份显示DBA结合减少或缺失，有2份显示PNA结合阳性。在溃疡性结肠炎的活动期，15份活检标本中有10份出现DBA结合缺失，而在缓解期全部恢复。1份重度发育异常的活检标本显示有PNA结合。结论是，正常结直肠黏膜与DBA强烈结合，但不与PNA结合。恶性组织和移行黏膜常常显示PNA结合，同时DBA结合减少。在溃疡性结肠炎中，DBA结合在活动期减少。
