Gratacós-Cubarsí M, Castellari M, Valero A, García-Regueiro J A
Institute for Food and Agricultural Research and Technology, Meat Technology Centre (IRTA-CTC), Granja Camps i Armet s/n, Monells, 17121, Girona, Spain.
Anal Bioanal Chem. 2006 Aug;385(7):1218-24. doi: 10.1007/s00216-006-0313-9. Epub 2006 Mar 14.
Use of an RP-C(12) analytical column with a mobile phase at pH 4.5 enabled excellent chromatographic separation and quantification of sulfadiazine (SDZ), sulfamerazine (SMR), and sulfamethazine (SMZ) in edible calf and pig tissue (muscle and liver). Tissue samples were extracted with acetonitrile, centrifuged without further clean-up, and analyzed by LC-DAD. The proposed conditions are useful for a rapid and reliable screening of the SDZ, SMR, and SMZ content of calf and pig tissue at concentrations lower than the maximum residue level (MRL) (LOQ between 27 and 55 ppb). Separation of some possible SMZ metabolites should be further investigated.
使用RP-C(12)分析柱，流动相pH值为4.5，能够对食用小牛和猪组织（肌肉和肝脏）中的磺胺嘧啶（SDZ）、磺胺甲基嘧啶（SMR）和磺胺二甲嘧啶（SMZ）进行出色的色谱分离和定量分析。组织样品用乙腈提取，离心后无需进一步净化，然后通过液相色谱-二极管阵列检测器（LC-DAD）进行分析。所提出的条件对于快速可靠地筛查小牛和猪组织中浓度低于最大残留限量（MRL）（定量限在27至55 ppb之间）的SDZ、SMR和SMZ含量很有用。一些可能的SMZ代谢物的分离还应进一步研究。
