Mor F, Sahindokuyucu Kocasari F, Ozdemir G, Oz B
University of Mehmet Akif Ersoy, Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology, 15030 Burdur, Turkey.
Bornova Veterinary Control and Research Institute, Toxicology Laboratory, Bornova, İzmir, Turkey.
Food Chem. 2012 Oct 1;134(3):1645-9. doi: 10.1016/j.foodchem.2012.03.049. Epub 2012 Mar 23.
The purpose of this study was determination of sulphonamide residues (sulphanilamide, sulphadiazine, sulphathiazole, sulphamerazine, sulphamethazine, sulphamethoxazole, and sulphadimethoxine) in cattle meat by the Charm II technique and the validation of sulphonamide levels by high performance liquid chromatography with fluorescence detector (HPLC-FLD). Of 157 meat samples, 9 samples (5.73%) were found positive by the Charm II method. To make quantitative confirmation of sulphonamide content of positive samples, HPLC-FLD was used and four samples were confirmed as positive. In HPLC analysis, the limit of detection (LOD) was in the range 8-15 μg/kg and the limit of quantification (LOQ) was 13-25 μg/kg. Average recoveries of sulphonamides ranged from 44.6% to 81% with relative standard deviations below 6% (n=6). In conclusion, we consider that the results obtained in field screening by only using the Charm II system, as is common practice in Turkey and worldwide, are inadequate and thus the results should be confirmed by sensitive systems like HPLC.
本研究的目的是采用Charm II技术测定牛肉中的磺胺类残留（磺胺、磺胺嘧啶、磺胺噻唑、磺胺甲基嘧啶、磺胺二甲嘧啶、磺胺甲恶唑和磺胺二甲氧嘧啶），并通过带荧光检测器的高效液相色谱法（HPLC-FLD）验证磺胺类药物的含量水平。在157份肉类样本中，Charm II方法检测出9份样本（5.73%）呈阳性。为了对阳性样本中的磺胺含量进行定量确认，采用了HPLC-FLD，其中4份样本被确认为阳性。在HPLC分析中，检测限（LOD）为8 - 15μg/kg，定量限（LOQ）为13 - 25μg/kg。磺胺类药物的平均回收率在44.6%至81%之间，相对标准偏差低于6%（n = 6）。总之，我们认为仅使用Charm II系统进行现场筛查所获得的结果（这在土耳其和全球都是常见做法）是不充分的，因此结果应由HPLC等灵敏系统进行确认。
