Kwon J M, Park Y H, Kang J H, Kim K, Ko Y H, Ryoo B Y, Lee S S, Lee S I, Koo H H, Kim W S
Division of Hematology/Oncology, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Ilwon-dong Kangnam-ku, 135-710, Seoul, South Korea.
Ann Hematol. 2006 Jul;85(7):463-8. doi: 10.1007/s00277-006-0081-9. Epub 2006 Mar 14.
The association of Epstein-Barr virus (EBV) with Hodgkin's lymphoma (HL) has been investigated over the last few years. The impact of EBV on clinical outcome is still controversial, however. In this study, we investigated the effect of EBV status on clinical outcome of HL patients. Between January 1986 and September 2004, fifty-six patients, diagnosed as having HL, were included in the analysis. Clinical data were reviewed retrospectively from the patients' records. Tissues from 56 patients were analyzed for the presence of EBV using the in situ hybridization (ISH) for EBV-encoded RNA (EBER) and immunohistochemistry for latent membrane protein (LMP)1. EBV infection was identified in 41.1% of cases by EBER ISH, 26.8% by LMP1 expression, and 26.8% by LMP1 and EBER ISH. EBER-positive HL were significantly more frequent in mixed cellularity (MC) subtype (P=0.014) and advanced stage (P=0.034). There was a trend toward shorter overall survival in EBER-positive patients without statistical significance (P=0.238). LMP1 expression also correlated with MC subtype (P=0.006) and advanced stage (P=0.007), although it did not significantly influence the survival outcome. In subgroup analysis, both EBER and LMP1 positivities were associated with longer progression-free survival in patients with age <25 years old (P=0.045). Reverse trends were shown in patients > or =25 years old. In this study, we demonstrated that the impact of tumor EBV status on prognosis may be age dependent and young patients with latent EBV infection have favorable prognosis.
在过去几年中，人们对爱泼斯坦-巴尔病毒（EBV）与霍奇金淋巴瘤（HL）之间的关联进行了研究。然而，EBV对临床结局的影响仍存在争议。在本研究中，我们调查了EBV状态对HL患者临床结局的影响。1986年1月至2004年9月期间，56例被诊断为HL的患者纳入分析。从患者记录中回顾性审查临床数据。使用针对EBV编码RNA（EBER）的原位杂交（ISH）和针对潜伏膜蛋白（LMP）1的免疫组织化学分析56例患者的组织中EBV的存在情况。通过EBER ISH在41.1%的病例中鉴定出EBV感染，通过LMP1表达鉴定出26.8%，通过LMP1和EBER ISH鉴定出26.8%。EBER阳性的HL在混合细胞性（MC）亚型（P = 0.014）和晚期（P = 0.034）中更为常见。EBER阳性患者的总生存期有缩短趋势，但无统计学意义（P = 0.238）。LMP1表达也与MC亚型（P = 0.006）和晚期（P = 0.007）相关，尽管它对生存结局没有显著影响。在亚组分析中，EBER和LMP1阳性均与年龄<25岁患者的无进展生存期延长相关（P = 0.045）。年龄≥25岁的患者呈现相反趋势。在本研究中，我们证明肿瘤EBV状态对预后的影响可能与年龄有关，潜伏性EBV感染的年轻患者预后良好。
