Guarnieri M
Lipids. 1975 May;10(5):294-8. doi: 10.1007/BF02532703.
Ca. 15% of the phosphatidyl inositol in myelin and microsomal membranes from rat brain was detectable by antiphosphatidyl inositol antibody. Antibody-detectable phosphatidyl inositol in myelin and microsomes readily increased when the membranes were incubated at 45 C with the antiserum. Synaptic membranes also had only a limited capacity to adsorb antibody. Quantitative binding studies with synaptic membranes and mitochondria were limited, because these membranes contain cardiolipin, which cross reacts with phosphatidyl inositol antisera. Moreover, highly purified synaptic and mitochondrial membranes contain appreciable amounts of other membrane fractions.
大鼠脑髓鞘和微粒体膜中约15%的磷脂酰肌醇可被抗磷脂酰肌醇抗体检测到。当髓鞘和微粒体膜在45℃与抗血清一起孵育时，抗体可检测到的磷脂酰肌醇很容易增加。突触膜吸附抗体的能力也有限。由于突触膜和线粒体含有与磷脂酰肌醇抗血清发生交叉反应的心磷脂，因此对它们进行的定量结合研究受到限制。此外，高度纯化的突触膜和线粒体膜含有相当数量的其他膜成分。
