人血小板膜从二酰基磷脂酰乙醇胺释放花生四烯酸。
Arachidonic acid release from diacyl phosphatidylethanolamine by human platelet membranes.
作者信息
Jesse R L, Cohen P
出版信息
Biochem J. 1976 Aug 15;158(2):283-7. doi: 10.1042/bj1580283.
At pH9.5 in the presence of 10 mM-Ca2+, human platelet membranes released 22% (167 of 785 nmol) of arachidonic acid that was esterified to phospholipids. With the use of synthetic choline (dinonadecanoyl) and ethanolamine (diheptadecanoyl) phosphoglycerides as internal reference compounds, 115 nmol of the released arachidonic acid was shown to be derived from endogenous breakdown of the phosphatidylethanolamine fraction. Further, the lysophosphatidylethanolamine that was released along with the arachidonic acid was shown virtually to lack fatty aldehydes and to contain a preponderance of fatty acids that have a preference for esterification at the 1-position of naturally occurring phosphatidylethanolamine of human platelets. These findings ruled out plasmalogen phosphatidylethanolamine as the source of the released arachidonic acid. We conclude that diacyl phosphatidylethanolamine was the principal source of arachidonic acid released by human platelet membranes under the conditions described.
在pH9.5以及存在10 mM钙离子的情况下，人血小板膜释放出22%（785纳摩尔中的167纳摩尔）与磷脂酯化的花生四烯酸。使用合成胆碱（二壬酰基）和乙醇胺（二庚酰基）磷酸甘油酯作为内参化合物，结果显示所释放的115纳摩尔花生四烯酸源自磷脂酰乙醇胺部分的内源性分解。此外，与花生四烯酸一同释放的溶血磷脂酰乙醇胺实际上显示缺乏脂肪醛，并且含有大量倾向于在人血小板天然存在的磷脂酰乙醇胺的1位酯化的脂肪酸。这些发现排除了缩醛磷脂酰乙醇胺作为所释放花生四烯酸的来源。我们得出结论，在所述条件下，二酰基磷脂酰乙醇胺是人血小板膜释放花生四烯酸的主要来源。
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