Tremblay Amy E, Buist Peter H, Hodgson Derek, Dawson Brian, Whittle Ed, Shanklin John
Department of Chemistry, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario, Canada K1S 5B6.
Magn Reson Chem. 2006 Jun;44(6):629-32. doi: 10.1002/mrc.1797.
1H-decoupled 19F NMR has been used to monitor the highly regioselective oxidation of a fluorine-tagged thia-fatty acid derivative by castor stearoyl-ACP delta9 desaturase. The major enzymatic product, after reductive work-up, was identified as 9-fluoro-1-nonanol. This compound could be easily distinguished from substrate and a 9-sulfoxy by-product on the basis of its 19F NMR chemical shift and spiking experiments using authentic standards. Structural assignment of the cleavage product was confirmed by GC-MS analysis of the enzymatic products.
1H 去耦 19F 核磁共振已被用于监测蓖麻硬脂酰 - ACP Δ9 去饱和酶对氟标记硫代脂肪酸衍生物的高区域选择性氧化。经过还原处理后，主要的酶促产物被鉴定为 9 - 氟 - 1 - 壬醇。基于其 19F 核磁共振化学位移以及使用标准品的加标实验，该化合物能够很容易地与底物和 9 - 亚磺酰氧基副产物区分开来。酶促产物的裂解产物的结构归属通过气相色谱 - 质谱联用分析得以证实。
