Appl Environ Microbiol. 1995 Apr;61(4):1658-60. doi: 10.1128/aem.61.4.1658-1660.1995.
Two laboratories tested four different brands of alkaline 2% glutaraldehyde sterilants by the Association of Official Analytical Chemists sporicidal test. Each laboratory found survival of Clostridium sporogenes spores on spore-labeled unglazed porcelain penicylinders (cylinders) to vary from test to test, and survival did not always correlate with increasing sterilant exposure time. These results were consistent with a theory that there may be random conditions within the test that prevent the sterilant from contacting all spores. Further studies indicated that the prior history of the unglazed porcelain cylinders and whether the C. sporogenes culture grown in egg-meat media had been processed (homogenized) to eliminate visible pieces of egg-meat media were important factors affecting the results and repeatability of this test.
两个实验室采用美国分析化学家协会（AOAC）的杀菌试验对四种不同品牌的碱性 2%戊二醛消毒剂进行了检测。每个实验室发现，梭状芽胞杆菌孢子在未上釉的瓷质钢笔（圆柱）上的存活情况因测试而异，而且存活情况并不总是与消毒剂暴露时间的增加相关。这些结果与一种理论一致，即测试中可能存在随机条件，阻止消毒剂接触所有孢子。进一步的研究表明，未上釉的瓷质圆柱的先前历史以及在蛋-肉培养基中生长的梭状芽胞杆菌培养物是否经过（匀浆）处理以消除可见的蛋-肉培养基碎片是影响该测试结果和重复性的重要因素。
