Appl Environ Microbiol. 1997 Aug;63(8):3304-7. doi: 10.1128/aem.63.8.3304-3307.1997.
The Association of Official Analytical Chemists (AOAC) test for sporicidal activity of disinfectants (966.04) is used in the United States as the legal criteria for classifying a liquid as a chemical sterilant and also as the indicator of the highest level of disinfectant. This qualitative test contains procedures that may cause inaccurate results. A modified, quantitative version of the AOAC sporicidal test has been developed that uses a specified minimum number of spores (2.0 x 10(sup5)) of Bacillus subtilis or Clostridium sporogenes dried onto porcelain penicylinders. This modified test was validated with three commercial sterilant chemicals tested on three separate groups of spore-labeled cylinders.
美国采用官方分析化学家协会（AOAC）的杀菌剂孢子杀灭活性测试（966.04）作为将液体分类为化学灭菌剂的法定标准，也是最高水平消毒剂的指标。该定性测试包含可能导致结果不准确的程序。已经开发出一种改良的、定量的 AOAC 孢子杀灭测试版本，该版本使用规定数量的枯草芽孢杆菌或梭状芽孢杆菌孢子（2.0 x 10(sup5))干燥到瓷质笔形管上。该改良测试使用三种商业灭菌化学品在三组单独的孢子标记管上进行了验证。
