Appl Environ Microbiol. 1995 Jul;61(7):2791-3. doi: 10.1128/aem.61.7.2791-2793.1995.
Denitrifying enzyme activity (DEA) in artificially established denitrification activity zones was, on average, 3.5-fold greater than in bulk soil. Denitrifier biomass was between 1 and 4 orders of magnitude greater in activity zones. Denitrifier specific activity was lower in activity zones and more similar to specific activity values reported for pure cultures of denitrifying bacteria added to soil. Measurements of DEA and biomass carried out on a homogeneous subsample derived from a soil core containing a denitrification activity zone will overestimate the DEA and biomass of the bulk soil and underestimate the DEA and biomass of the activity zone.
人工建立的反硝化活性区中的反硝化酶活性（DEA）平均比原状土壤高 3.5 倍。反硝化菌生物量在活性区中高出 1 到 4 个数量级。反硝化菌的比活度在活性区中较低，与添加到土壤中的纯培养反硝化细菌的比活度值更为相似。对取自含有反硝化活性区的土壤芯的均匀亚样本进行的 DEA 和生物量测量，会高估原状土壤的 DEA 和生物量，低估活性区的 DEA 和生物量。
