生物反应器培养的灰色链霉菌 CBS 109.60 中的土腥素和相关挥发物。
Geosmin and Related Volatiles in Bioreactor-Cultured Streptomyces citreus CBS 109.60.
出版信息
Appl Environ Microbiol. 1996 Apr;62(4):1295-9. doi: 10.1128/aem.62.4.1295-1299.1996.
Abstract
Streptomyces citreus CBS 109.60 produced geosmin and a complex pattern of other volatile compounds during cultivation in a 2.5-liter laboratory bioreactor. Volatiles were isolated from disrupted cells, from the culture medium, and from the waste air of the bioreactor by adsorption on Lewatit OC 1064MD. Quantitative and qualitative analyses were carried out using capillary gas chromatography and coupled gas chromatography-mass spectroscopy. S. citreus produced 56 volatile compounds, which were mainly terpenoids but also included aliphatic ketones, alcohols, esters, pyrazines, furan(on)es, and aromatic types during the growth phase. The major components were geosmin and a germacradienol. A biosynthetic pathway for geosmin including eudesmanolides is proposed.
摘要
柠檬色链霉菌 CBS 109.60 在 2.5 升实验室生物反应器中培养时会产生土臭素和一系列其他挥发性化合物。通过Lewatit OC 1064MD 吸附，从破碎细胞、培养基和生物反应器废气中分离出挥发性物质。使用毛细管气相色谱法和气相色谱-质谱联用进行定量和定性分析。柠檬色链霉菌在生长阶段产生了 56 种挥发性化合物，主要为萜烯类化合物，但也包括脂肪族酮、醇、酯、吡嗪、呋喃（on）和芳香族化合物。主要成分是土臭素和一种倍半萜烯醇。提出了包括艾杜烷内酯在内的土臭素生物合成途径。
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