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Appl Environ Microbiol. 1996 Jun;62(6):2152-5. doi: 10.1128/aem.62.6.2152-2155.1996.
2
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本文引用的文献

1
Improved production of Trichoderma harzianum endochitinase by expression in Trichoderma reesei.通过在里氏木霉中表达提高哈茨木霉内切几丁质酶的产量。
Appl Environ Microbiol. 1996 Jun;62(6):2145-51. doi: 10.1128/aem.62.6.2145-2151.1996.
2
Isolation and sequence of an endochitinase-encoding gene from a cDNA library of Trichoderma harzianum.从哈茨木霉cDNA文库中分离并测序一个编码内切几丁质酶的基因。
Gene. 1994 Jan 28;138(1-2):143-8. doi: 10.1016/0378-1119(94)90797-8.
3
Characterization of ech-42, a Trichoderma harzianum endochitinase gene expressed during mycoparasitism.哈茨木霉内切几丁质酶基因ech-42的特性分析，该基因在真菌寄生过程中表达。
Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):10903-7. doi: 10.1073/pnas.91.23.10903.
4
Parallel formation and synergism of hydrolytic enzymes and peptaibol antibiotics, molecular mechanisms involved in the antagonistic action of Trichoderma harzianum against phytopathogenic fungi.哈茨木霉对植物病原真菌拮抗作用中涉及的水解酶和肽抗生素的平行形成与协同作用及分子机制
Appl Environ Microbiol. 1994 Dec;60(12):4364-70. doi: 10.1128/aem.60.12.4364-4370.1994.
5
Efficient production of antibody fragments by the filamentous fungus Trichoderma reesei.丝状真菌里氏木霉高效生产抗体片段
Biotechnology (N Y). 1993 May;11(5):591-5. doi: 10.1038/nbt0593-591.
6
Cloning and characterization of a chitinase (chit42) cDNA from the mycoparasitic fungus Trichoderma harzianum.哈茨木霉几丁质酶（chit42）cDNA的克隆与特性分析
Curr Genet. 1994 Dec;27(1):83-9. doi: 10.1007/BF00326583.
7
Production of recombinant proteins in the filamentous fungus Trichoderma reesei.在丝状真菌里氏木霉中生产重组蛋白。
Curr Opin Biotechnol. 1995 Oct;6(5):534-7. doi: 10.1016/0958-1669(95)80088-3.
8
A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei.一种用于纤维素分解丝状真菌里氏木霉的通用转化系统。
Gene. 1987;61(2):155-64. doi: 10.1016/0378-1119(87)90110-7.
9
Regulation of chitinase synthesis in Trichoderma harzianum.哈茨木霉中几丁质酶合成的调控
J Gen Microbiol. 1991 Sep;137(9):2163-9. doi: 10.1099/00221287-137-9-2163.
10
Biomimics of fungal cell-cell recognition by use of lectin-coated nylon fibers.利用凝集素包被的尼龙纤维对真菌细胞间识别进行仿生模拟。
J Bacteriol. 1992 Feb;174(3):1055-9. doi: 10.1128/jb.174.3.1055-1059.1992.

用里氏木霉 cbh1 启动子增强哈茨木霉中的内切几丁质酶表达。

Enhanced Expression of Endochitinase in Trichoderma harzianum with the cbh1 Promoter of Trichoderma reesei.

出版信息

Appl Environ Microbiol. 1996 Jun;62(6):2152-5. doi: 10.1128/aem.62.6.2152-2155.1996.

DOI:10.1128/aem.62.6.2152-2155.1996
PMID:16535342
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1388880/
Abstract

Production of extracellular endochitinase could be increased 5-fold in the mycoparasite fungus Trichoderma harzianum by using the cellulase promoter cbh1 of Trichoderma reesei, whereas the total endochitinase activity increased 10-fold. The cbh1 promoter was not expressed on glucose and sucrose in T. harzianum and was induced by sophorose and on cellulase-inducing medium. The endogenous endochitinase gene was expressed at a low basal level on glucose and sucrose. No specific induction by crab shell chitin or sophorose was observed.

摘要

通过使用里氏木霉的纤维素酶启动子 cbh1，可使生防真菌哈茨木霉的胞外内切几丁质酶产量增加 5 倍，而总内切几丁质酶活性增加 10 倍。在哈茨木霉中，cbh1 启动子在葡萄糖和蔗糖上不表达，而是被槐糖和纤维素诱导型培养基诱导表达。内源性内切几丁质酶基因在葡萄糖和蔗糖上的基础表达水平较低。未观察到壳聚糖或槐糖的特异性诱导。