Keränen S, Penttilä M
VTT Biotechnology and Food Research, Espoo, Finland.
Curr Opin Biotechnol. 1995 Oct;6(5):534-7. doi: 10.1016/0958-1669(95)80088-3.
The potential of the filamentous fungus Trichoderma reesei for producing heterologous proteins has recently been demonstrated with a number of secreted proteins. Rate-limiting steps and ways of improving the production have been studied, especially using antibody Fab fragments. Major improvements have been achieved by producing the foreign protein fused to the fungal cellulase cellobiohydrolase I. In addition to the strong inducible cbh1 promoter, other promoters, which are regulated in a different manner, have been developed.
丝状真菌里氏木霉生产异源蛋白的潜力最近已通过多种分泌蛋白得到证实。人们已经研究了限速步骤和提高产量的方法，特别是使用抗体Fab片段的情况。通过生产与真菌纤维素酶纤维二糖水解酶I融合的外源蛋白，已取得了重大进展。除了强诱导型cbh1启动子外，还开发了其他以不同方式调控的启动子。
