Appl Environ Microbiol. 1996 Nov;62(11):4202-5. doi: 10.1128/aem.62.11.4202-4205.1996.
The distribution of symbiotic (Sym) plasmid types across background genotypes was investigated in two field populations of Rhizobium leguminosarum biovar viciae isolated directly from soils. PCR-based methods were used to characterize the background genotypes and the Sym gene types. Identical Sym gene types were associated with a variable range of background genotypes, while the same background genotype could harbor distinct Sym gene types. Random distributions of Sym gene types in the background genotypes were observed in the two soil populations. These results suggest that Sym plasmid transfer is less restricted than previously thought on the basis of the analysis of strains isolated from legume nodules.
本研究直接从土壤中分离的两个根瘤菌属豌豆根瘤菌生物变种田间种群中,调查了共生(Sym)质粒类型在背景基因型中的分布。采用基于 PCR 的方法对背景基因型和 Sym 基因类型进行了特征分析。相同的 Sym 基因类型与不同的背景基因型相关联,而相同的背景基因型则可以携带不同的 Sym 基因类型。在两个土壤种群中,观察到 Sym 基因类型在背景基因型中的随机分布。这些结果表明,Sym 质粒的转移比以前基于从豆科植物根瘤中分离的菌株分析所认为的要不受限制。