碲和硒抗性在根瘤菌中的表现及其在直接从土壤中分离苜蓿根瘤菌中的潜在应用。
Tellurium and Selenium Resistance in Rhizobia and Its Potential Use for Direct Isolation of Rhizobium meliloti from Soil.
机构信息
Soil and Water Management Research Unit, Agricultural Research Service, U. S. Department of Agriculture, St. Paul, Minnesota 55108.
出版信息
Appl Environ Microbiol. 1994 May;60(5):1674-7. doi: 10.1128/aem.60.5.1674-1677.1994.
Abstract
Forty-eight Rhizobium and Bradyrhizobium strains were screened for resistance to tellurite, selenite, and selenate. High levels of resistance to the metals were observed only in Rhizobium meliloti and Rhizobium fredii strains; the MICs were 2 to 8 mM for Te(IV), >200 mM for Se(VI), and 50 to 100 mM for Se(IV). Incorporation of Se and Te into growth media permitted us to directly isolate R. meliloti strains from soil. Mutant strains of rhizobia having decreased levels of Se and Te resistance were constructed by Tn5 mutagenesis and were found to have transposon insertions in DNA fragments of different sizes. Genomic DNAs from Te rhizobium strains failed to hybridize with Te determinants from plasmids RP4, pHH1508a, and pMER610.
摘要
对 48 株根瘤菌和慢生根瘤菌进行了抗亚碲酸盐、硒酸盐和亚硒酸盐的筛选。只有在根瘤菌属和根瘤菌属菌株中观察到对这些金属的高水平抗性；对 Te(IV)的 MIC 为 2 至 8 mM，对 Se(VI)的 MIC 为>200 mM，对 Se(IV)的 MIC 为 50 至 100 mM。将硒和碲掺入生长培养基中，使我们能够直接从土壤中分离出根瘤菌属菌株。通过 Tn5 诱变构建了具有降低的 Se 和 Te 抗性的根瘤菌突变株，并发现它们在大小不同的 DNA 片段中有转座子插入。来自 Te 根瘤菌菌株的基因组 DNA 与来自质粒 RP4、pHH1508a 和 pMER610 的 Te 决定簇不杂交。
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