Appl Environ Microbiol. 1997 Feb;63(2):567-73. doi: 10.1128/aem.63.2.567-573.1997.
L-Arogenate is a commonplace amino acid in nature in consideration of its role as a ubiquitous precursor of L-phenylalanine and/or L-tyrosine. However, the questions of whether it serves as a chemoattractant molecule and whether it can serve as a substrate for catabolism have never been studied. We found that Pseudomonas aeruginosa recognizes L-arogenate as a chemoattractant molecule which can be utilized as a source of both carbon and nitrogen. Mutants lacking expression of either cyclohexadienyl dehydratase or phenylalanine hydroxylase exhibited highly reduced growth rates when utilizing L-arogenate as a nitrogen source. Utilization of L-arogenate as a source of either carbon or nitrogen was dependent upon (sigma)(sup54), as revealed by the use of an rpoN null mutant. The evidence suggests that catabolism of L-arogenate proceeds via alternative pathways which converge at 4-hydroxyphenylpyruvate. In one pathway, prephenate formed in the periplasm by deamination of L-arogenate is converted to 4-hydroxyphenylpyruvate by cyclohexadienyl dehydrogenase. The second route depends upon the sequential action of periplasmic cyclohexadienyl dehydratase, phenylalanine hydroxylase, and aromatic aminotransferase.
L-精氨酸是一种常见的氨基酸，考虑到它作为 L-苯丙氨酸和/或 L-酪氨酸的普遍前体的作用。然而，它是否作为趋化吸引分子起作用，以及它是否可以作为分解代谢的底物，这些问题从未被研究过。我们发现铜绿假单胞菌将 L-精氨酸识别为趋化吸引分子，它可以作为碳和氮的来源。当利用 L-精氨酸作为氮源时，缺乏环已二烯基脱水酶或苯丙氨酸羟化酶表达的突变体表现出极低的生长速率。L-精氨酸作为碳或氮源的利用依赖于（西格玛）（sup54），这是通过使用 rpoN 缺失突变体揭示的。证据表明，L-精氨酸的分解代谢通过在 4-羟基苯丙酮酸处汇聚的替代途径进行。在一条途径中，由 L-精氨酸脱氨基在周质中形成的预苯酸盐被环己二烯基脱氢酶转化为 4-羟基苯丙酮酸。第二条途径取决于周质中环己二烯基脱水酶、苯丙氨酸羟化酶和芳香族氨基转移酶的顺序作用。
