低溶解氧浓度对嗜碱真养产碱杆菌聚（3-羟基丁酸-co-3-羟基戊酸）生产的影响。
Effects of Low Dissolved-Oxygen Concentrations on Poly-(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Production by Alcaligenes eutrophus.
出版信息
Appl Environ Microbiol. 1997 Mar;63(3):827-33. doi: 10.1128/aem.63.3.827-833.1997.
Abstract
The bacterial copolyester poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) was produced with Alcaligenes eutrophus DSM 545 from glucose and sodium propionate in a fed-batch fermentation with both nitrogen limitation and low dissolved-oxygen concentrations. When the dissolved-oxygen content was kept between 1 and 4% of air saturation during the polymer accumulation phase, the yield of 3-hydroxybutyrate (3HB) monomer from glucose was not affected, but the propionate-to-3-hydroxyvalerate (3HV) monomer yield was two to three times (0.48 to 0.73 mol of 3HV mol of propionate consumed(sup-1)) that observed in a control experiment (0.25 mol mol(sup-1)), where the accumulation-phase dissolved-oxygen concentration was 50 to 70% of air saturation. The overall polymer productivity of the fermentation was somewhat decreased by low dissolved-oxygen contents, owing to a slower 3HB production rate. The effect of a low dissolved-oxygen concentration is probably attributable to a reduction of the oxygen-requiring decarbonylation of propionyl-coenzyme A (CoA) to acetyl-CoA.
摘要
细菌共聚酯聚（3-羟基丁酸酯-co-3-羟基戊酸酯）是由葡萄糖和丙酸钠在补料分批发酵中，利用氮限制和低溶解氧浓度，由 Alcaligenes eutrophus DSM 545 生产的。当聚合物积累阶段的溶解氧含量保持在空气饱和度的 1%至 4%之间时，葡萄糖中 3-羟基丁酸（3HB）单体的产率不受影响，但丙酸与 3-羟基戊酸（3HV）单体的产率是对照实验（0.25 mol mol(sup-1))中观察到的两倍到三倍（0.48 至 0.73 mol 3HV mol 消耗的丙酸（sup-1))，其中积累阶段的溶解氧浓度为空气饱和度的 50%至 70%。由于 3HB 生产速率较慢，低溶解氧含量会使发酵的整体聚合物生产力略有下降。低溶解氧浓度的影响可能归因于减少了需要氧气的丙酰辅酶 A（CoA）脱羧化为乙酰辅酶 A。
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本文引用的文献
1
Biosynthesis of Poly-beta-Hydroxyalkanoates from Pentoses by Pseudomonas pseudoflava.假单胞菌属从戊糖合成聚-β-羟基烷酸酯。
Appl Environ Microbiol. 1990 Oct;56(10):3133-8. doi: 10.1128/aem.56.10.3133-3138.1990.
2
Production of a polyhydroxyalkanoate biopolymer in insect cells with a modified eucaryotic fatty acid synthase.利用修饰的真核脂肪酸合酶在昆虫细胞中生产聚羟基脂肪酸酯生物聚合物。
Appl Environ Microbiol. 1996 Jul;62(7):2540-6. doi: 10.1128/aem.62.7.2540-2546.1996.
3
Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high levels of polymer accumulation.将聚羟基丁酸酯生物合成途径靶向拟南芥的质体导致聚合物高水平积累。
Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):12760-4. doi: 10.1073/pnas.91.26.12760.
4
Scale-up of a cyclone bioreactor.气旋生物反应器的放大
J Chem Technol Biotechnol. 1994 Jan;59(1):83-9. doi: 10.1002/jctb.280590113.
5
The role of oxygen limitation in the formation of poly- -hydroxybutyrate during batch and continuous culture of Azotobacter beijerinckii.氧限制在拜氏固氮菌分批培养和连续培养过程中聚羟基丁酸酯形成中的作用
Biochem J. 1972 Aug;128(5):1193-201. doi: 10.1042/bj1281193.
6
Cloning and expression in Escherichia coli of the Alcaligenes eutrophus H16 poly-beta-hydroxybutyrate biosynthetic pathway.嗜碱假单胞菌H16聚-β-羟基丁酸酯生物合成途径在大肠杆菌中的克隆与表达。
J Bacteriol. 1988 Oct;170(10):4431-6. doi: 10.1128/jb.170.10.4431-4436.1988.
7
Cloning of the Alcaligenes eutrophus genes for synthesis of poly-beta-hydroxybutyric acid (PHB) and synthesis of PHB in Escherichia coli.用于合成聚-β-羟基丁酸（PHB）的嗜中性产碱杆菌基因的克隆及在大肠杆菌中合成PHB
J Bacteriol. 1988 Dec;170(12):5837-47. doi: 10.1128/jb.170.12.5837-5847.1988.
8
Production of poly-(beta-hydroxybutyric-co-beta-hydroxyvaleric) acids.聚（β-羟基丁酸-co-β-羟基戊酸）的生产
Appl Environ Microbiol. 1990 Jul;56(7):2093-8. doi: 10.1128/aem.56.7.2093-2098.1990.
9
Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates.细菌聚羟基脂肪酸酯的产生、代谢、代谢作用及工业用途
Microbiol Rev. 1990 Dec;54(4):450-72. doi: 10.1128/mr.54.4.450-472.1990.
10
Materials derived from biomass/biodegradable materials.源自生物质/可生物降解材料的材料。
Proc Natl Acad Sci U S A. 1992 Feb 1;89(3):839-42. doi: 10.1073/pnas.89.3.839.
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