3-羟基丁酸和3-羟基戊酸的可生物降解共聚酯在真养产碱杆菌中的积累。

Accumulation of biodegradable copolyesters of 3-hydroxy-butyrate and 3-hydroxyvalerate in Alcaligenes eutrophus.

作者信息

Chua H, Yu P H, Lo W

机构信息

Department of Civil and Structural Engineering, Hong Kong Polytechnic University, Hung Hom.

出版信息

Appl Biochem Biotechnol. 1998 Spring;70-72:929-35. doi: 10.1007/BF02920203.

DOI:10.1007/BF02920203

PMID:9627404

Abstract

Biodegradable copolyesters of 3-hydroxybutyrate-co-3-hydroxyvalerate (3HB-3HV) were produced by Alcaligenes eutrophus in a two-staged process, namely growth stage and nitrogen-deficient polyester-accumulation stage. When C5 was used as the sole carbon source, the copolyester contained 43 mol % of 3HV. A range of copolyesters with 0-43 mol % of 3HV could be produced by using a medium containing different concentration ratios of butyric acid C4 and C5. Tm of PHB homopolymer was 177.6 degrees C and that of copolyester with highest 3HV mol fraction of 43% was 99.0 degrees C. C5 concentration in the medium could be an effective means to control the polymeric composition and mechanical properties of the copolyesters accumulated in A. eutrophus.

摘要

3-羟基丁酸酯-3-羟基戊酸酯（3HB-3HV）的生物可降解共聚酯由真养产碱杆菌通过两阶段过程生产，即生长阶段和缺氮聚酯积累阶段。当使用C5作为唯一碳源时，该共聚酯含有43摩尔%的3HV。通过使用含有不同浓度比的丁酸C4和C5的培养基，可以生产出一系列含有0-43摩尔% 3HV的共聚酯。聚-3-羟基丁酸酯（PHB）均聚物的熔点为177.6℃，3HV摩尔分数最高为43%的共聚酯的熔点为99.0℃。培养基中的C5浓度可能是控制真养产碱杆菌中积累的共聚酯的聚合物组成和机械性能的有效手段。