Appl Environ Microbiol. 1997 May;63(5):1749-55. doi: 10.1128/aem.63.5.1749-1755.1997.
The white rot fungus Bjerkandera sp. strain BOS55 extensively delignified and bleached oxygen-delignified eucalyptus kraft pulp handsheets. Biologically mediated brightness gains of up to 14 ISO (International Standards Organization units) were obtained, providing high final brightness values of up to 80% ISO. In nitrogen-limited cultures (2.2 mM N), manganese (Mn) greatly improved manganese-dependent peroxidase (MnP) production. However, the biobleaching was not affected by the Mn nutrient regimen, ranging from 1,000 (mu)M added Mn to below the detection limit of 0.26 (mu)M Mn in EDTA-extracted pulp medium. The lowest Mn concentration tested was at least several orders of magnitude lower than the K(infm) known for MnP. Consequently, it was concluded that Mn is not required for biobleaching in Bjerkandera sp. strain BOS55. Nonetheless, fast protein liquid chromatography profiles indicated that MnP was the predominant oxidative enzyme produced even under culture conditions in the near absence of manganese. High nitrogen (22 mM N) and exogenous veratryl alcohol (2 mM) repressed biobleaching in Mn-deficient but not in Mn-sufficient culture medium. No correlation was observed between the titers of extracellular peroxidases and the biobleaching. However, the decolorization rate of the polyaromatic dye Poly R-478 was moderately correlated to the biobleaching under a wide range of Mn and N nutrient regimens.
白腐真菌 Bjerkandera sp. 菌株 BOS55 可有效降解木质素并漂白氧脱木质素桉木硫酸盐浆手抄片。通过生物介导可获得高达 14 ISO（国际标准化组织单位）的增白效果，从而使最终白度值高达 80% ISO。在氮限制培养（2.2 mM N）中，锰（Mn）极大地促进了锰依赖过氧化物酶（MnP）的产生。然而，生物漂白不受 Mn 营养方案的影响，Mn 的添加浓度范围从 1000 μM 到 EDTA 提取纸浆培养基中检测不到的 0.26 μM Mn。测试的最低 Mn 浓度至少比已知的 MnP 的 K(infm)低几个数量级。因此，可以得出结论，Mn 不是 Bjerkandera sp. 菌株 BOS55 生物漂白所必需的。尽管如此，快速蛋白质液相色谱图谱表明，即使在 Mn 缺乏的培养条件下，MnP 也是主要产生的氧化酶。高氮（22 mM N）和外加藜芦醇（2 mM）抑制了 Mn 缺乏但 Mn 充足的培养基中的生物漂白。在外源过氧化物酶的效价与生物漂白之间未观察到相关性。然而，在广泛的 Mn 和 N 营养方案下，多环芳烃染料 Poly R-478 的脱色率与生物漂白呈中度相关。
