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云芝新型细胞外过氧化物酶编码cDNA的克隆与特性分析

Cloning and characterization of a cDNA encoding a novel extracellular peroxidase from Trametes versicolor.

作者信息

Collins P J, O'Brien M M, Dobson A D

机构信息

Microbiology Department, University College Cork, Cork, Ireland.

出版信息

Appl Environ Microbiol. 1999 Mar;65(3):1343-7. doi: 10.1128/AEM.65.3.1343-1347.1999.

Abstract

The white rot basidiomycete Trametes versicolor secretes a large number of peroxidases which are believed to be involved in the degradation of polymeric lignin. These peroxidases have been classified previously as lignin peroxidases or manganese peroxidases (MnP). We have isolated a novel extracellular peroxidase-encoding cDNA sequence from T. versicolor CU1, the transcript levels of which are repressed by low concentrations of Mn2+ and induced by nitrogen and carbon but not induced in response to a range of stresses which have been reported to induce MnP expression.

摘要

白腐担子菌云芝分泌大量过氧化物酶,据信这些酶参与聚合木质素的降解。这些过氧化物酶先前已被归类为木质素过氧化物酶或锰过氧化物酶(MnP)。我们从云芝CU1中分离出了一个新的细胞外过氧化物酶编码cDNA序列,其转录水平受到低浓度Mn2+的抑制,受氮和碳诱导,但对一系列据报道可诱导MnP表达的胁迫无诱导反应。

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本文引用的文献

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Regulation of Laccase Gene Transcription in Trametes versicolor.
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