Gabriel Matthias, Nazmi Kamran, Veerman Enno C, Nieuw Amerongen Arie V, Zentner Andrej
Departments of Orthodontics, Academic Centre for Dentistry Amsterdam (ACTA), The Netherlands.
Bioconjug Chem. 2006 Mar-Apr;17(2):548-50. doi: 10.1021/bc050091v.
Modification of material surfaces aimed at bestowing them with antimicrobial properties is a promising approach in the development of new biomaterials. Antimicrobial peptides (AMPs) are an attractive alternative to conventional antibiotics because of lack of toxicity, inherently high selectivity, and absence of immune response. As the antimicrobial mode of action of the AMP cathelin LL37 is formation of pores and disruption of microbial membrane, the purpose of the present study was to develop and test a method of covalent immobilization of LL37 on titanium surface. The application of a flexible hydrophilic poly(ethylene glycol) spacer and selective N-terminal conjugation of LL37 resulted in a surface peptide layer which was capable of killing bacteria on contact.
旨在赋予材料表面抗菌性能的材料表面改性是新型生物材料开发中一种很有前景的方法。抗菌肽（AMPs）是传统抗生素的一种有吸引力的替代品，因为它们无毒、具有固有的高选择性且不会引起免疫反应。由于抗菌肽cathelin LL37的抗菌作用方式是形成孔道并破坏微生物膜，本研究的目的是开发并测试一种将LL37共价固定在钛表面的方法。使用柔性亲水性聚乙二醇间隔物以及LL37的选择性N端共轭，形成了一个能够在接触时杀死细菌的表面肽层。
