Capocchi Antonella, Fontanini Debora, Muccilli Vera, Cunsolo Vincenzo, Saviozzi Franco, Saletti Rosaria, Foti Salvatore, Galleschi Luciano
Department of Botanical Sciences, University of Pisa, via L. Ghini 5, 56126 Pisa, Italy.
J Agric Food Chem. 2006 Mar 22;54(6):2372-7. doi: 10.1021/jf0524731.
Nonspecific lipid-transfer proteins (nsLTPs) have been recognized as allergens in several plant species among which are cereals important in human nutrition. In this report, we purified a 9600 +/- 1 Da protein from both soft wheat and farro bran. Mass spectrometric analyses revealed that these proteins are identical, belong to the nsLTP1 class, and have high sequence homology with nsLTP1 isolated from other cereal species. Their identification was further supported by the ability of the soft wheat nsLTP1 to transfer pyrene-labeled lipids between donor and acceptor membranes. The results are discussed in view of the increasing diffusion on the markets of bran-rich products.
非特异性脂质转移蛋白（nsLTPs）已被确认为几种植物物种中的过敏原，其中包括对人类营养至关重要的谷物。在本报告中，我们从软质小麦和二粒小麦麸皮中纯化出一种9600±1 Da的蛋白质。质谱分析表明，这些蛋白质是相同的，属于nsLTP1类别，并且与从其他谷物物种中分离出的nsLTP1具有高度的序列同源性。软质小麦nsLTP1在供体膜和受体膜之间转移芘标记脂质的能力进一步支持了它们的鉴定。鉴于富含麸皮产品在市场上的日益普及，对这些结果进行了讨论。
