Pelillo Marco, Iafelice Giovanna, Marconi Emanuele, Fiorenza Caboni Maria
Dipartimento di Scienze degli Alimenti, Università di Bologna, via Ravennate 1020, 47023 Cesena, Italy.
Rapid Commun Mass Spectrom. 2003;17(20):2245-52. doi: 10.1002/rcm.1156.
Free sterols from hexaploid and tetraploid free-threshing wheats (Triticum aestivum L. and T. durum Desf.) and from their respective hulled wheats (T. spelta L. and T. dicoccon Schrank) were analysed by gas chromatography with mass spectrometry. The qualitative analysis of sterols showed a similar pattern either between hexaploid (T. aestivum, T. spelta) and tetraploid (T. durum, T. dicoccon) wheats or between free-threshing (T. aestivum, T. durum) and hulled (T. spelta, T. dicoccon) wheats. However, quantitative differences were found between tetraploid and hexaploid wheats, in that free sterol amounts in tetraploid wheats were 40% higher than in hexaploid ones. The mass spectra of the sterols were classified into four groups, taking into account the structural features of rings A and B. Typical mass spectral fragmentations of the four classes, and additional evidence related to the side chain of each molecule, were investigated together with their chromatographic behaviour, allowing identification of all the detected sterols.
采用气相色谱-质谱联用技术对六倍体和四倍体普通小麦（Triticum aestivum L.和T. durum Desf.）及其各自的带壳小麦（T. spelta L.和T. dicoccon Schrank）中的游离甾醇进行了分析。甾醇的定性分析表明，六倍体（T. aestivum、T. spelta）和四倍体（T. durum、T. dicoccon）小麦之间，或者普通小麦（T. aestivum、T. durum）和带壳小麦（T. spelta、T. dicoccon）之间，甾醇模式相似。然而，四倍体和六倍体小麦之间存在数量差异，四倍体小麦中的游离甾醇含量比六倍体小麦高40%。根据A环和B环的结构特征，将甾醇的质谱分为四类。研究了这四类典型的质谱裂解以及与每个分子侧链相关的其他证据，并结合其色谱行为，从而鉴定出所有检测到的甾醇。
