Pedrero Zoyne, Madrid Yolanda, Cámara Carmen
Departamento de Química Analítica, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, Ciudad Universitaria s/n, 28040 Madrid Spain.
J Agric Food Chem. 2006 Mar 22;54(6):2412-7. doi: 10.1021/jf052500n.
An in vitro gastrointestinal method was employed to predict the potential bioavailability of selenium and its species from radish, belonging to the Brassicaceae family, grown in hydroponics media in the presence of inorganic selenium, such as Na2SeO3 and Na2SeO4. A low transformation of Se into organic forms was observed in radish plants grown in Se(VI)-enriched culture media. On the contrary, in those plants exposed to selenite, >95% of the total selenium was found as selenocystine (SeCys2), selenomethionine (SeMet), and Se-methylselenocysteine (SeMetSeCys). The concentrations of these species in fresh samples remained almost unaltered after a simulated gastrointestinal digestion. Therefore, a high selenium content of Se-methylselenocysteine (65%), previously reported as a cancer chemopreventive species, remained in the potentially bioabsorbable fraction. As these plants usually undergo a short development cycle, these results suggest that radish enriched in selenite could be a good choice as an organoselenium supplement for the human diet and animal feed.
采用体外胃肠道方法预测在水培介质中添加无机硒（如亚硒酸钠和硒酸钠）种植的十字花科萝卜中硒及其形态的潜在生物利用度。在富含硒（VI）的培养基中生长的萝卜植株中，观察到硒向有机形态的转化率较低。相反，在暴露于亚硒酸盐的植株中，总硒的>95%以硒代胱氨酸（SeCys2）、硒代蛋氨酸（SeMet）和硒甲基硒代半胱氨酸（SeMetSeCys）的形式存在。经过模拟胃肠道消化后，新鲜样品中这些形态的浓度几乎保持不变。因此，先前报道具有癌症化学预防作用的硒甲基硒代半胱氨酸（65%）的高硒含量仍保留在潜在可生物吸收部分。由于这些植物通常生长周期较短，这些结果表明，富含亚硒酸盐的萝卜可能是人类饮食和动物饲料中有机硒补充剂的良好选择。
