Goldsmith D J, Tribe R M, Poston L, Cappuccio F P, Markandu N D, MacGregor G A, Hilton P J
Renal Research Laboratory, St Thomas' Hospital, London, UK.
J Hypertens. 1991 Jul;9(7):645-53. doi: 10.1097/00004872-199107000-00010.
The cellular basis for essential hypertension remains obscure. Abnormal ion transport has been demonstrated in both experimental and essential hypertension, raised levels of sodium-lithium (Na(+)-Li+) and sodium-proton (Na(+)-H+) exchange in blood cells being a consistent feature. However, Na(+)-H+ exchange is not the main regulator of intracellular pH at resting pH, while the importance of the contribution of bicarbonate to cellular pH regulation is now increasingly appreciated. Serum and serum-derived growth factors are known to affect intracellular pH and the activity of the Na(+)-H+ antiporter. This study was designed to investigate the activity of Na(+)-H+ exchange in the leucocytes of patients with essential hypertension in the presence of bicarbonate in vitro and to measure the effect of autologous serum on intracellular pH and Na(+)-H+ exchange. Paired serum samples from essential hypertensives and their controls were used on leucocytes from other (unrelated, normotensive) donors to investigate the same parameters. In a study of 30 patients with untreated essential hypertension and 30 controls matched for age, sex, race and body habitus we found no difference in resting pH or buffering capacity (pH 7.28 +/- 0.01 and 32.0 +/- 1.6 mmol/l per pH, hypertensives, versus 7.27 +/- 0.02 and 34.5 +/- 1.8 mmol/l per pH, controls) but a marked difference in the maximal rate of Na(+)-H+ exchange in response to intracellular acidification (57.8 +/- 3.2 mmol/l per min versus 47.2 +/- 1.4 mmol/l per min, P = 0.004).(ABSTRACT TRUNCATED AT 250 WORDS)
原发性高血压的细胞基础仍不清楚。在实验性高血压和原发性高血压中均已证实存在离子转运异常，血细胞中钠 - 锂（Na(+)-Li+）和钠 - 质子（Na(+)-H+）交换水平升高是一个一致的特征。然而，在静息pH值时，Na(+)-H+交换并非细胞内pH的主要调节因子，而现在人们越来越认识到碳酸氢盐对细胞pH调节作用的重要性。已知血清和血清源性生长因子会影响细胞内pH以及Na(+)-H+反向转运体的活性。本研究旨在体外碳酸氢盐存在的情况下，研究原发性高血压患者白细胞中Na(+)-H+交换的活性，并测量自体血清对细胞内pH和Na(+)-H+交换的影响。使用原发性高血压患者及其对照的配对血清样本，对来自其他（无关的、血压正常的）供体的白细胞进行检测，以研究相同的参数。在一项针对30例未经治疗的原发性高血压患者和30例年龄、性别、种族和体型相匹配的对照的研究中，我们发现静息pH或缓冲能力无差异（高血压患者分别为7.28±0.01和每pH值32.0±1.6 mmol/l，对照组分别为7.27±0.02和每pH值34.5±1.8 mmol/l），但在细胞内酸化后，Na(+)-H+交换的最大速率存在显著差异（分别为57.8±3.2 mmol/l每分钟和47.2±1.4 mmol/l每分钟，P = 0.004）。（摘要截断于250字）
