McManus B L, Fuller B W, Boetel M A, French B W, Ellsbury M M, Head G P
Plant Science Department, Northern Plains Biostress Laboratory, South Dakota State University, Brookings, SD 57007, USA.
J Econ Entomol. 2005 Dec;98(6):1992-8. doi: 10.1603/0022-0493-98.6.1992.
Lady beetles (Coleoptera: Coccinellidae) are important polyphagous predators in maize, Zea mays L., fields. Transgenic Cry3Bb1 maize hybrids express a coleopteran-specific insecticidal protein derived from Bacillus thuringiensis (Berliner) subsp. kumamotoensis that is targeted at corn rootworm larvae. This study evaluated impacts of Cry3Bb1 protein-expressing maize, tefluthrin-treated maize, and untreated controls on lady beetle abundance at preanthesis, anthesis, and postanthesis maize-developmental periods near Brookings in eastern South Dakota during 2001 and 2002. The dominant lady beetle species captured on Pherocon AM sticky traps was Coleomegilla maculata De Geer. It comprised 73.5 and 69.9% of all adult Coccinellidae caught in 2001 and 2002, respectively. Numbers of C. maculata captured in Cry3Bb1 maize were not significantly different from those in untreated plots during preanthesis, and adults were more abundant in Cry3Bb1 maize than in tefluthrin-treated and untreated plots during anthesis and postanthesis. Whole-plant sampling confirmed C. maculata predominance with the species representing 89.2 and 91.4% of all adult lady beetles observed in 2001 and 2002, respectively. Whole-plant sampling also indicated a lack of negative effects from Cry3Bb1 maize on abundance of lady beetle eggs, larvae, pupae, or adults. Overall, these findings indicate that Cry3Bb1-expressing hybrids are not likely to impose harmful effects on C. maculata, a species common to maize production systems in the northern Great Plains. This research further suggests that Cry3Bb1 maize has the potential for conservation of these beneficial coccinellids in maize production systems.
瓢虫（鞘翅目：瓢虫科）是玉米田（玉米，Zea mays L.）中重要的多食性捕食者。转基因Cry3Bb1玉米杂交种表达一种源自苏云金芽孢杆菌（Berliner）熊本亚种的鞘翅目特异性杀虫蛋白，该蛋白针对玉米根虫幼虫。本研究评估了在2001年和2002年南达科他州东部布鲁金斯附近，表达Cry3Bb1蛋白的玉米、氟虫腈处理的玉米和未处理的对照在玉米发育的抽雄前期、花期和花后期对瓢虫数量的影响。在Pherocon AM粘虫板上捕获的优势瓢虫种类是黄斑瓢虫Coleomegilla maculata De Geer。它分别占2001年和2002年捕获的所有成年瓢虫科昆虫的73.5%和69.9%。在抽雄前期，Cry3Bb1玉米中捕获的黄斑瓢虫数量与未处理地块中的数量没有显著差异，在花期和花后期，Cry3Bb1玉米中的成虫比氟虫腈处理和未处理地块中的更丰富。全株采样证实黄斑瓢虫占优势，该物种分别占2001年和2002年观察到的所有成年瓢虫的89.2%和91.4%。全株采样还表明，Cry3Bb1玉米对瓢虫卵、幼虫、蛹或成虫的数量没有负面影响。总体而言，这些发现表明，表达Cry3Bb1的杂交种不太可能对黄斑瓢虫产生有害影响，黄斑瓢虫是大平原北部玉米生产系统中的常见物种。这项研究进一步表明，Cry3Bb1玉米在玉米生产系统中具有保护这些有益瓢虫的潜力。
