Martin J D, Li P N
Mercer University School of Medicine, Macon, GA 31207.
Virus Res. 1991 May;19(2-3):163-72. doi: 10.1016/0168-1702(91)90043-u.
The genomic regulatory regions of human isolates of SV40 were compared by nucleotide sequencing and by biological assays of their transcriptional enhancers. SVAST is a defective genome and SVMEN an infectious genome molecularly cloned from human astrocytoma and meningioma, respectively (Krieg and Scherer, 1984). SVPML is an isolate from a case of progressive multifocal leukoencephalopathy (Weiner et al., 1972). SVAST contains a duplication of the regulatory region. One copy is virtually identical to that of SV40; the other bears a deletion of the minimal enhancer and is associated with an insertion of early/late sequences derived from the terminus of replication. The latter copy, together with its insertion, is inserted at nucleotide 4966 (SV40 number). SVMEN has an almost exact deletion of one of the 72-base-pair repeats of the natural SV40 enhancer. The regulatory sequences of SVPML were reported earlier (Martin, 1989). The enhancer activity in CV-1 cells of the lytic virus, SVPML, is 36% that of SV40. In contrast, the SVAST and SVMEN enhancers are more effective, being equal to or 64% that of SV40, respectively. The possibility that SVPML and SVAST/SVMEN originated from different variants of SV40 is discussed.
通过核苷酸测序以及对其转录增强子进行生物学分析，对人源SV40分离株的基因组调控区域进行了比较。SVAST是一种缺陷型基因组，而SVMEN是分别从人星形细胞瘤和脑膜瘤分子克隆得到的感染性基因组（克里格和谢勒，1984年）。SVPML是从一例进行性多灶性白质脑病患者分离得到的毒株（韦纳等人，1972年）。SVAST包含调控区域的重复。其中一个拷贝与SV40的几乎完全相同；另一个拷贝则缺失了最小增强子，并与源自复制末端的早期/晚期序列插入有关。后一个拷贝及其插入序列插入在核苷酸4966处（SV40编号）。SVMEN几乎精确缺失了天然SV40增强子的一个72碱基对重复序列。SVPML的调控序列此前已有报道（马丁，1989年）。裂解性病毒SVPML在CV - 1细胞中的增强子活性是SV40的36%。相比之下，SVAST和SVMEN的增强子更有效，分别相当于SV40的增强子活性或为其64%。文中讨论了SVPML和SVAST/SVMEN源自SV40不同变体的可能性。
